Fig. 3

In vitro stage conversion assay. A Representative fluorescence images of amylopectin-containing WT and Δpgm1 cysts at 4 days post-induction. Infected monolayers were stained with PAS to detect amylopectin (red), DBA to label the cyst wall (green), and DAPI for nuclei (blue); Scale bar = 10 microns. B Representative images of WT and Δpgm1 cysts 4 days post-induction in vitro. The images are representative of the mean value of cyst areas for each strain. Cysts were stained with DBA (red), anti-GRA7 (green), and DAPI (blue); Scale bar = 10 microns. C Quantification of cyst sizes. The areas of 176 WT and 185 Δpgm1 cysts were determined in pixels² at 4 days post-induction from 3 independent experiments; *p = 0.0362 by nonparametric Mann–Whitney test