Fig. 1
From: The G1/S repressor WHI5 is expressed at similar levels throughout the cell cycle
WHI5 mRNA is detected across all cell cycle stages. A Projections of the sN&B time-series of the same cells shown on Additional file 2: Video S1, Additional file 3: Video S2, Additional file 4: Video S3, Additional file 5: 4 Video S4, using a different intensity scale allowing for the visualization of cells from auto-fluorescence only in absence of 2x-yeGFP plasmid. Red arrows indicate exemplary unbudded G1 cells showing clear WHI5 mRNA spots in Whi5mRNA-24x-yeGFP cells exclusively. B Cell-averaged yeGFP stoichiometry (vertical axis) as a function of cell-averaged yeGFP fluorescence intensity (horizontal axis) in individual single yeGFP cells (yellow dots), Whi5-PP7tag cells (blue dots) and Whi5mRNA-24x-yeGFP cells (green dots). Each dot represents an individual cell. yeGFP stoichiometry was identified to yeGFP molecular brightness (see details in Additional file 1), obtained from the analysis of sN&B data similar to (and including) data shown in A. The raw quantitative data underlying B are provided in Additional file 1