Fig. 2
From: The G1/S repressor WHI5 is expressed at similar levels throughout the cell cycle
WHI5 mRNA is expressed in similar amount across all cell cycle stages. A Top: example images of Whi5mRNA-24x-yeGFP (sample) cells (left), and control yeGFP cells (right). Scale bar: 5 µm. Examples of putative WHI5 mRNA spots and artifactual yeGFP bright spots are indicated with green and red arrows respectively. Bottom: distribution of the number of WHI5 mRNA detections per cell as determined by confocal microscopy in small unbudded (blue), large unbudded/small-budded (orange) and medium-to-large budded (grey) Whi5mRNA-24x-yeGFP cells (left, N = 488, 435, 79 cells in the 3 cell cycle stages respectively), and yeGFP control cells (right, N = 445, 416, 107 cells in the 3 cell cycle stages respectively). B Example images showing WHI5 mRNA molecules localization in single cells (left) relative to the nucleus, as determined by Hoechst 33342 staining (middle, merged images: right). WHI5 mRNA molecules were found both in the cytosol (red arrows) and the nucleus (green arrows). Images are representative of hundreds of similar cells. Scale bar: 5 µm. The larger size of some WHI5 mRNA spots in these experiments may be attributable to the longer time lag between the imaging of two z-stack planes (due to the exposure time of the Hoechst 33342 dye), during which mRNA molecules diffuse, to a higher excitation power, or to the presence of several WHI5 mRNA molecules within the same nuclei. The raw quantitative data underlying A are provided in Additional file 1