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Fig. 1 | BMC Research Notes

Fig. 1

From: Gel shift experiments with fragments of the Trypanosoma brucei RNA-binding protein RBP10

Fig. 1

Production of recombinant proteins. A Cartoon showing RBP10 and produced fragments. F2 and F8 were expressed from pET24a ( +) and F7 from pQEA38. B Coomassie-blue stained SDS-polyacrylamide gel showing purification of His-RBP10. Equal proportions of lysates from induced and uninduced cells, sonicated extracts and the soluble and insoluble fractions, are shown. Equal volumes of eluate fractions are also shown. C Purification of F2 and F8 proteins. Details are as in B except that samples from dialyzed eluates are also illustrated. D Purification of F7 protein. Details are as in B

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