Fig. 2From: Gel shift experiments with fragments of the Trypanosoma brucei RNA-binding protein RBP10Gel shifts obtained using purified RBP10 and fragment F2. A Sequences of the 26mer probe (EP1) and the mutant version (EP1-m), which is inactive in developmental regulation, both used at 500Â pM. B Gel shift with full-length RBP10, added in progressive twofold dilutions. The asterisk shows a smear of bound RNAs. C Gel shift with RBP10 fragment F2, added in progressive twofold dilutions. D Gel shift with RBP10 fragment F8, added in progressive twofold dilutions. E Gel shift with RBP10 fragment F7, added in progressive twofold dilutionsBack to article page