Characterization of polymorphic microsatellite markers for the Japanese endangered land snail Mandarina

Nishida, Shu; Mimura, Kotaro; Mori, Hideaki; Chiba, Satoshi

doi:10.1186/s13104-022-06147-4

BMC Research Notes

Table 1 Characterization of the polymorphic microsatellite markers for Mandarina mandarina

From: Characterization of polymorphic microsatellite markers for the Japanese endangered land snail Mandarina

Locus	Primer sequence (5'–3')	Repeat motif	Size range (bp)	Na	Ho	He	Fis	Pid	Pid (sib)
Manman_12260B	F: AGCGTTACATACGTCACTTTGG R: ACGAATAGTGGTGTCTACCCG	CTG	230–287	15	0.708	0.906	0.218	0.016	0.30
Manman_161603	F: TGCGGTCTTATTTCATTGCAG R: CAACAGATAGGCTTGCCGTG	TTC	168–189	8	0.625	0.837	0.253	0.047	0.34
Manman_247740	F: TCATAGTGACTGCACATGCC R: TGTTCAAACGATTTCCAAAAAGC	TAA	227–269	13	0.522	0.889	0.413	0.023	0.31
Manman_392524	F: GGTTTAAGACGATGGCACGG R: TTAATTCTTTAACAACGGCAAGC	ATG	249–279	10	0.696	0.846	0.178	0.042	0.34
Manman_378362	F: TCAGAACTATTCCTGTTGTGTGC R: ATTAAACTGCAAGCGGTTCG	TC	174–206	14	0.708	0.896	0.209	0.019	0.31
Manman_6672	F: GAGCAGTATCATCCCCTTGC R: CACGCCCAGAAAATAAAAACTATCG	TCA	242–278	13	0.783	0.894	0.125	0.020	0.31
Manman_31975	F: CGAGGCATCTCCAAAAACAC R: TTATGGGTCTGCCCCGAG	TGA	105–123	6	0.375	0.498	0.247	0.380	0.57
Manman_308475	F: TCATGACCTCTGTCCTATGCC R:TCTCGAGTTGACTGTTTAGTATCATC	ATG	154–193	13	0.583	0.885	0.341	0.239	0.31
Manman_1709	F: ATAGAATGGGGTCGTGCCTC R: TCGACGCTGACAAGCAAATG	TTC	230–317	20	0.792	0.927	0.151	0.011	0.29
Manman_7787A	F: GATATGCTGTTGGAGGTCGC R: TGTCTGTTAATTGCCCACGC	TGA	257–326	16	0.583	0.876	0.381	0.025	0.32
Manman_30938	F: GGCTTTGTTTTGTAGCACCG R: ACTTTGTGATCTCCCAGTCG	GTTA	227–279	14	0.625	0.885	0.293	0.024	0.31
Manman_118744	F: ACTATGACGCACCATCTCCC R: GAGTCAACTTGCCCATCACAG	GAT	118–148	11	0.667	0.799	0.165	0.060	0.37
Manman_235472	F: GGTTCTATGGGGTTTACAGCG R: TCCATATACTGACATTGTTTGCC	TAT	267–336	15	0.625	0.908	0.312	0.016	0.30
Manman_46250	F: CATGGCGAACCCTAATCTGC R: TGGGTCTGACTATATGAGGGC	AG	175–207	14	0.667	0.871	0.234	0.028	0.32
Manman_82352B	F: AGAACAACGGGGTACTGGAG R: GCTTGCGTGTCCGATTTTTAAC	GA	223–285	17	0.708	0.905	0.217	0.016	0.30
Manman_4034†	F: CGCTTCGGTCTAGAATGCAC R: ACTCCATCTTAGCCTCTGTCG	AG	221–261	15	0.750	0.917	0.182	0.013	0.30
Manman_209567*	F: AGGCATGTTTTCTTTTTAGATAATGG R: TATTGTGCACTGAGCTCGAC	GA	263–299	14	0.542	0.879	0.384	0.026	0.32
Manman_345444*	F: ATCGTACCAGACATCTGCCC R: GGCTGTCAGGGCCATTATTC	GA	160–206	16	0.696	0.878	0.208	0.023	0.32
Manman_13329A*	F: TGTACACGAGCTTTTCGACC R: CGATGCACGAATTGAGTCCC	GA	128–152	11	0.500	0.865	0.422	0.032	0.33
Manman_140288*	F: CGCGTTCCCCAATACTCAAC R: ACAGTGTCTGAAACCGGTAG	TTC	204–276	20	0.625	0.919	0.320	0.012	0.29
Manman_183849*	F: GGCTACGGCATAAGGTGAG R: GGAATACATCCTGCAAGCGG	GAT	168–207	11	0.333	0.870	0.617	0.031	0.32
Manman_82799*	F: CACCAGGAGTCGCAAATGAC R: ACTGCCATCAGGTTGTGAAG	AG	121–183	18	0.458	0.923	0.503	0.011	0.29
Manman_90631*	F: TAGATGTGGTGGAGGCATCG R: CCCTTAGCGTATCTCCACAGG	GA	242–302	16	0.478	0.907	0.473	0.016	0.30

Na the number of alleles, Ho observed heterozygosity, He expected heterozygosity, Fis inbreeding coefficient. PID probability of identity, PID (sib) probability of siblings
A dagger ^†indicates that loci were not clearly amplified in multiplex PCR
An asterisk ^*indicates that loci showed deviation from hardy–Weinberg equilibrium (P < 0.05)

Back to article page

ISSN: 1756-0500

Contact us

Submission enquiries: bmcresearchnotes@biomedcentral.com
General enquiries: ORSupport@springernature.com