Fig. 1

CB nanoparticle characterization and deposition in lung. A CB particle characterization table and real-time mass concentration measurements (mg/m³) of the CB aerosols during an inhalation exposure for 6 h for a target concentration of 6 mg/m³. Data represent an average of three exposures. B Particle size distribution of the carbon black aerosol measured from the exposure chamber using a high resolution electrical low-pressure impactor (ELPI +). A log-normal fit of the distributions resulted in a count median diameter (CMD) of 67 nm with a geometric standard deviation (GSD) of 2.13 nm. Inset: representative image of CB particles. Scale bar 500 nm. C Particle size distribution of the CB aerosol measured from the exposure chamber with a cascade impactor (Nano-MOUDI). A log-normal fit of the distribution resulted in a mass median aerodynamic diameter of 975 nm with a GSD of 2.47 nm. D TEM and SEM images of CB agglomerate collected on a TEM grid from the exposure 6 mg/m³ chamber