Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study

Table 2 Mean difference of group with their confidence interval and p-value following heat inactivation at different temperatures and durations EPHI, Ethiopia

Group	Groups	MD (95%CI)	P-value
T1	T1 vs T2	0.10 (0.02 to 0.18)	0.01
	T1 vs T3	0.15 (0.10 to 0.24)	0.001
	T1 vs Bulk lysis	0.13 (0.02 to 0.24)	0.02
T2	T2 vs T1	− 0.1 (− 0.20 to − 0.02)	0.01
	T2 vs T3	0.05 (− 0.01 to 0.11)	0.16
	T2 vs Bulk lysis	0.03 (− 0.01 to 0.13)	0.999
T3	T3 vs T1	− 0.15 (− 0.24 to − 0.1)	0.001
	T3 vs T2	− 0.05 (− 0.11 to 0.01)	0.16
	T3 vs Bulk lysis	− 0.02 (− 0.12 to 0.10)	0.999
Bulk lysis	T1	− 0.13 (− 0.24 to − 0.20)	0.02
	T2	− 0.03 (− 0.13 to 0.10)	0.999
	T3	0.02 (− 0.10 to 0.12)	0.999

MD mean in difference, CI Confidence interval, T1 temperature at 56 °C, T2 temperature at 60 °C, T3 temperature at 100 °C

ISSN: 1756-0500