Fig. 1

CRISPR/Cas9-induced knockout mutations in QPT2_T and QPT2_S. The 20 bp sequence targeted by the nuclease is underlined and the PAM site is shown in red type. A Alignment of tobacco QPT2 and QPT1 genes in the region of the target site. The 3 bp deletion and two single nucleotide polymorphisms that are found within the comparable 20 bp region of QPT1_T and QPT1_S are highlighted in blue. Other polymorphisms among the QPT genes are highlighted in gray. Gene mutations found in the three lines that were characterized in the greenhouse and field are shown in B. Inserted nucleotides and the positions of deleted nucleotides (dashes) are represented in green type. The numbers in black type correspond to the QPT cDNA sequences beginning at the start ATG codon; orange numbers represent amino acid position