Fig. 2

Growth characteristics and alkaloid content of greenhouse-grown tobacco plants possessing knockout mutations in QPT2 genes. Blue bar graphs show data comparisons among mutant lines T8 and T21 and their corresponding TN90 WT control; green bar graphs depict the K19 mutant and its K326 WT control. Measurements include: plant height (A), leaf number (B), leaf weight (C), flowering time (D), nicotine (E) and nornicotine (F). For flowering time, days = days post-transplant and transfer to the greenhouse. Means and standard error bars are shown above each genotype. Asterisks indicate significant differences between qpt2 mutant lines and their corresponding WT control at P < 0.05 (*), P < 0.01 (**) and P < 0.001 (***) as determined by t-tests. BLOQ, below level of quantification