Table 1 Primers used in the study
Primer | Oligonucleotide sequence (5’–3’) | Tm (°C) | Product (bp) | References |
|---|---|---|---|---|
rec -F rec -R | TGGTCAAGCTCAGCACCAAT TACACAAGCCAGACGGTTCC | 67 | 361 | This study |
β-globin | ACACAACTGTGTTCACTAGC CAACTTCATCCACGTTCACC | 50 | 110 | [14] |
Primer | Oligonucleotide sequence (5’–3’) | Tm (°C) | Product (bp) | References |
|---|---|---|---|---|
rec -F rec -R | TGGTCAAGCTCAGCACCAAT TACACAAGCCAGACGGTTCC | 67 | 361 | This study |
β-globin | ACACAACTGTGTTCACTAGC CAACTTCATCCACGTTCACC | 50 | 110 | [14] |