The impact of Hyssop (Hyssopus officinalis) extract on activation of endosomal toll like receptors and their downstream signaling pathways

Table 1 Gene and protein expression of TLRs and downstream molecules in studied population

Target	Control (mean ± SD) (N = 45)	After 12 h (mean ± SD) (N = 45)	After 24 h (mean ± SD) (N = 45)	After 48 h (mean ± SD) (N = 45)	p value
Target	Control (mean ± SD) (N = 45)	After 12 h (mean ± SD) (N = 45)	After 24 h (mean ± SD) (N = 45)	After 48 h (mean ± SD) (N = 45)	Control vs. After 12 h	Control vs. After 24 h	Control vs. After 48 h
Relative gene expression (Fold change)
Myd88	1.001 ± 0.08261	1.188 ± 0.5632	1.599 ± 0.4567	1.667 ± 0.6941	NS	< 0.0001	< 0.0001
NFқB	1.000 ± 0.07271	1.060 ± 0.5233	1.218 ± 0.6694	1.395 ± 0.4382	NS	NS	< 0.0001
TLR-3	1.000 ± 0.09613	1.220 ± 0.6738	1.371 ± 0.8069	1.746 ± 1.184	NS	0.0110	0.0004
TLR-7	1.000 ± 0.1076	1.294 ± 0.8883	1.652 ± 1.055	1.860 ± 0.7372	NS	0.0005	< 0.0001
TLR-8	1.000 ± 0.06735	1.090 ± 0.2698	1.416 ± 0.8921	1.463 ± 0.7341	NS	0.0094	0.0004
TLR-9	1.000 ± 0.1192	1.059 ± 0.3625	1.226 ± 0.5289	1.673 ± 1.075	NS	0.0226	0.0004
Enzyme-linked immunosorbent assay (pg/ml)
IFN-Iα	89.56 ± 34.96	102.2 ± 47.01	120.1 ± 52.12	140.1 ± 57.64	NS	0.0048	< 0.0001
IFN-Iβ	95.31 ± 30.82	111.4 ± 38.57	132.7 ± 49.70	147.9 ± 52.39	NS	0.0002	< 0.0001
IL-1β	64.91 ± 33.20	56.36 ± 28.35	47.02 ± 26.06	40.07 ± 24.50	NS	0.0167	0.0004
TNF-α	36.87 ± 19.50	30.44 ± 15.55	25.84 ± 12.09	22.71 ± 10.46	NS	0.0057	0.0002
IL-8	121.8 ± 44.87	107.8 ± 43.71	94.51 ± 42.57	89.56 ± 38.08	NS	0.0118	0.0012

MyD88 Myeloid differentiation primary response 88, NFκB Nuclear factor kappa B, TLRs Toll-Like receptors, IFN-Is Type I interferons; ILs: Interleukins, TNF-α Tumor necrosis factor alpha. Data are presented as mean ± standard division (SD). P < 0.05 was considered as statistically significant

ISSN: 1756-0500