Fig. 2
The inhibitory components of P. fluorescens CS are heat-resistant and not small molecules. A P. fluorescens CS was boiled for various times up to 5 min before treating S. epidermidis. B P. fluorescens CS was treated with proteinase K and used for S. epidermidis biofilm. C Boiled P. fluorescens CS was filtered in a Millipore Centricon tube with 3 K MW limit (> 3 K MW: retentate; < 3 K MW: filtrate). D S. epidermidis 1457 biofilm assay was performed with or without 25% CS. The preformed biofilms were treated with NaIO4 for disruption. Data shown as means from three separate assays. The bars indicate standard deviation (n = 9). **, *** indicate statistical significance at P < 0.01 and P < 0.001, respectively, ns: not significant