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Fig. 2 | BMC Research Notes

Fig. 2

From: Clinically relevant benzoxaboroles inhibit mRNA processing in Trypanosoma brucei

Fig. 2

Effect of acoziborole and AN11736 benzoxaboroles on mRNA processing. A T. brucei bloodstream forms were treated with acoziborole for 10 h. RNA was extracted before adding the compound (0-hour lane) and at the time points indicated on the blots. The RNA was analysed by denaturing gel electrophoresis and Northern blotting. a The blots were first stained with methylene blue as loading control. b The blot was probed with [32P]-labelled oligonucleotide complementary to the spliced leader (SL) to detect spliced mRNAs and the 140-nt spliced leader-containing precursor, SLRNA. c The blot was stripped and hybridised with a 32P-labelled probe specific to the mRNA encoding β-tubulin (β-TUB). B As (A) but with AN11763 treatment

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