Fig. 2From: Clinically relevant benzoxaboroles inhibit mRNA processing in Trypanosoma brucei Effect of acoziborole and AN11736 benzoxaboroles on mRNA processing. A T. brucei bloodstream forms were treated with acoziborole for 10 h. RNA was extracted before adding the compound (0-hour lane) and at the time points indicated on the blots. The RNA was analysed by denaturing gel electrophoresis and Northern blotting. a The blots were first stained with methylene blue as loading control. b The blot was probed with [32P]-labelled oligonucleotide complementary to the spliced leader (SL) to detect spliced mRNAs and the 140-nt spliced leader-containing precursor, SLRNA. c The blot was stripped and hybridised with a 32P-labelled probe specific to the mRNA encoding β-tubulin (β-TUB). B As (A) but with AN11763 treatmentBack to article page