Fig. 3
From: Clinically relevant benzoxaboroles inhibit mRNA processing in Trypanosoma brucei
Effect of AN7973 on CPSF73-TAP expression. A Growth curve (cumulative cell numbers) showing the viability of cells expressing only TAP-tagged CPSF73 (CPSF73-TAP), or having only a single copy of the CPSF73 gene (SKO). Cells were counted daily and diluted to maintain densities below 2 × 106/ml. wt = starting cell line. B Dose response curves showing the EC50 of AN7973 for CPSF73-TAP SKO cells in comparison to wildtype cells. Three technical replicates were analysed. C Western blot showing expression of CPSF73-TAP after incubating the cells with AN7973 for 6 h. The values below the bands are the amount of CPSF73-TAP after treatment relative to no treatment. Image below the western blot is ponceau red stain and was used as a loading control