Fig. 1

Comparison of Mannheimia haemolytica species-specificity of lktD and rsmL LAMP primers. Colorimetric LAMP reactions were performed with lktD (a) and rsmL (b) primer sets using a pH-based colorimetric LAMP kit (phenol red) with the purified genomic DNA (5 ng) from M. haemolytica genotypes 1 and 2 strains and four related Pasteurellaceae species at 65 °C for 60 min. A positive reaction is indicated by a color change from pink to yellow. LAMP amplicons obtained with lktD (c) and rsmL (d) primers were further analyzed by agarose gel electrophoresis. Tubes/lanes information: 1. M. haemolytica genotype 2 (ST1, D153); 2. genotype 1 (ST2, D171); 3. genotype 2 (ST6, D174); 4. Bibersteinia trehalosi; 5. Mannheimia glucosida, 6. Pasteurella multocida; 7. Histophilus somni; 8. No template control. In each gel, molecular weight marker (Bioline HyperLadder 1 kb) is shown to the left of lane 1