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Fig. 3 | BMC Research Notes

Fig. 3

From: Differential identification of Mannheimia haemolytica genotypes 1 and 2 using colorimetric loop-mediated isothermal amplification

Fig. 3

Colorimetric LAMP to determine the sensitivity of Mannheimia haemolytica genotype-specific primers. Sensitivity of LAMP primer sets designed for M. haemolytica genotype 1 (a, adhesin pseudogene B1, strain 22604 [ST2]) and genotype 2 (b, c; adhesin G, strains 32635 [ST1] and 33041 [ST6]) were performed using a non-pH-based multi-purpose LAMP kit supplemented with hydroxynaphthol blue with 10 × serially diluted genomic DNA at 65 °C for 60 min. A positive reaction is indicated by a color change from violet to sky blue. LAMP amplicons obtained with adhesin pseudogene B1 and adhesin G were also analyzed by agarose gel electrophoresis (d). Tubes/lanes information: 1. genotype 1 [ST2] 105 copies; 2. 104 copies; 3. 103 copies; 4. 102 copies; 5. 101 copies; 6. 100 copies; 7. No template control; 8. genotype 2 [ST1] 105 copies; 9. 104 copies; 10. 103 copies; 11. 102 copies; 12. 101 copies; 13. 100 copies; 14. No template control; 15. genotype 2 [ST6] 105 copies; 16. 104 copies; 17. 103 copies; 18. 102 copies; 19. 101 copies; 20. 100 copies; 21. No template control. In each gel, molecular weight marker (Bioline HyperLadder 1 kb) is shown at left to the lane 1, 8 or 15, respectively

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