Fig. 1

Ixodes scapularis removed from dogs were received from locations across southern Ontario and tested via real-time PCR for B. burgdorferi (positive = red triangle, negative = yellow circle). Positive samples were further analyzed to determine MLST sequence type (labels associated with red triangles). Longitude and latitude of tick location were based on owner-reported suspected location of tick acquisition. If a location was described but it was not specific (i.e., ‘backyard’), the tick was geocoded to the submitting veterinary clinic. Samples for which the location of tick acquisition could not be determined (e.g., missing data, numerous locations listed) have not been visually projected