Table 2 Primer sequences for cDNA synthesis and real‑time PCR.
MiRNAs and their accession numbers | Sequences | ||
|---|---|---|---|
cDNA Synthesis reaction | hsa-mir-887-5p (MIMAT0004949) | hsa-miR-877-5p(STL) | 5ʹGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCTGC3ʹ |
hsa-mir-583 (MIMAT0003248) | hsa-mir-583(STL) | 5ʹGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTAATGG3ʹ | |
RNU6 NR_003027.2 | U6(STL) | 5′GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAATAT3ʹ | |
Real time PCR reaction | hsa-mir-887-5p (MIMAT0004949) | hsa-miR-877-5p(F) | 5′GTAGAGGAGATGGCGCAGGG3′ |
hsa-mir-583 (MIMAT0003248) | hsa-mir-583(F) | 5′CCCAAAGAGGAAGGTCCCATTAC3′ | |
RNU6 NR_003027.2 | U6(F) U6(R) | 5′GCTTCGGCAGCACATATACTAAAAT3′ 5′CGCTTCACGAATTTGCGTGTCAT3′ | |
hsa-mir-877-5p and hsa-mir-583 | Common(R) | 5′GTGCAGGGTCCGAGGT3′ | |