Articles

Studies of the transmissibility of the agent of bovine spongiform encephalopathy to the domestic chicken

Transmission of the prion disease bovine spongiform encephalopathy (BSE) occurred accidentally to cattle and several other mammalian species via feed supplemented with meat and bone meal contaminated with infe...

Authors: Jo Moore, Stephen AC Hawkins, Anthony R Austin, Timm Konold, Robert B Green, Ian W Blamire, Ian Dexter, Michael J Stack, Melanie J Chaplin, Jan PM Langeveld, Marion M Simmons, Yvonne I Spencer, Paul R Webb, Michael Dawson and Gerald AH Wells

Clinicopathological, therapeutic and prognostic features of the triple-negative tumors in moroccan breast cancer patients (experience of Hassan II university hospital in Fez)

Triple-negative breast cancer (TNBC) is defined as a group of breast carcinomas that are negative for expression of hormone receptors (ER, PR) and Her2, we can distinguish between two groups: basal-like (ER-, ...

Authors: Yousra Akasbi, Sanae Bennis, Fouad Abbass, Kawtar Znati, Khalid Amrani Joutei, Afaf Amarti and Omar EL Mesbahi

Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen

Studies of nuclear function in many organisms, especially those with tough cell walls, are limited by lack of availability of simple, economical methods for large-scale preparation of clean, undamaged nuclei.

Authors: Robert M Givens, Larry D Mesner, Joyce L Hamlin, Michael J Buck and Joel A Huberman

Effect of haemolysis and repeated freeze-thawing cycles on wild boar serum antibody testing by ELISA

Monitoring wildlife diseases is needed to identify changes in disease occurrence. Wildlife blood samples are valuable for this purpose but are often gathered haemolysed. To maximise information, sera often go ...

Authors: Mariana Boadella and Christian Gortázar

Histological analysis of surgical lumbar intervertebral disc tissue provides evidence for an association between disc degeneration and increased body mass index

Although histopathological grading systems for disc degeneration are frequently used in research, they are not yet integrated into daily care routine pathology of surgical samples. Therefore, data on histopath...

Authors: Christoph Weiler, Mercedes Lopez-Ramos, H Michael Mayer, Andreas Korge, Christoph J Siepe, Karin Wuertz, Veronique Weiler, Norbert Boos and Andreas G Nerlich

Waterpipe smoking among health sciences university students in Iran: perceptions, practices and patterns of use

In recent years waterpipe smoking has become a popular practice amongst young adults in eastern Mediterranean countries, including Iran. The aim of this study was to assess waterpipe smoking perceptions and pr...

Authors: Nasim Ghafouri, Jan D Hirsch, Gholamreza Heydari, Candis M Morello, Grace M Kuo and Renu F Singh

Cook It Up! A community-based cooking program for at-risk youth: overview of a food literacy intervention

In Canada, there are limited occasions for youth, and especially at-risk youth, to participate in cooking programs. The paucity of these programs creates an opportunity for youth-focused cooking programs to be...

Authors: Heather MC Thomas and Jennifer D Irwin

Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma

Up-regulation of S100A7 (Psoriasin), a small calcium-binding protein, is associated with the development of several types of carcinomas, but its function and possibility to serve as a diagnostic or prognostic ...

Authors: Manuela R Barbieri, Camillo DC Andrade, Wilson A Silva Jr, Adriana A Marques, Andréia M Leopoldino, Marlise BA Montes, Marcelo Dias-Baruffi, Iberê C Soares, Alda Wakamatsu, Venâncio AF Alves, Hélen J Laure, Marco A Zago and Lewis J Greene

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

Polygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing...

Authors: Erik Alexandersson, John VW Becker, Dan Jacobson, Eric Nguema-Ona, Cobus Steyn, Katherine J Denby and Melané A Vivier

Impulsivity and pathological gambling: Is it a state or a trait problem?

This study tested 37 Chinese male pathological gamblers and 40 controls to understand the relationship between pathological gambling and impulsivity as a long-term trait or a short-term state in the cognitive ...

Authors: Florence DM Lai, Alison KY Ip and Tatia MC Lee

Self medication with antibiotics in Yogyakarta City Indonesia: a cross sectional population-based survey

Self medication with antibiotics has become an important factor driving antibiotic resistance. This study investigated the period prevalence, patterns of use, and socio-demographic factors associated with self...

Authors: Aris Widayati, Sri Suryawati, Charlotte de Crespigny and Janet E Hiller

Myoblasts generated by lentiviral mediated MyoD transduction of myotonic dystrophy type 1 (DM1) fibroblasts can be used for assays of therapeutic molecules

Myotonic dystrophy type 1 (DM1) is the most common muscle dystrophy in adults. The disease is caused by a triplet expansion in the 3'end of the myotonic dystrophy protein kinase (DMPK) gene. In order to develop a...

Authors: Jan Larsen, Olof J Pettersson, Maria Jakobsen, Rune Thomsen, Christina B Pedersen, Jens M Hertz, Niels Gregersen, Thomas J Corydon and Thomas G Jensen

Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

The def mutant pea (Pisum sativum L) showed non-abscission of seeds from the funicule. Here we present data on seed development and growth pattern and their relationship in predicting this particular trait in wil...

Authors: Kwadwo Owusu Ayeh, YeonKyeong Lee, Mike J Ambrose and Anne Kathrine Hvoslef-Eide

Variability of protein level and phosphorylation status caused by biopsy protocol design in human skeletal muscle analyses

Bergström needle biopsy is widely used to sample skeletal muscle in order to study cell signaling directly in human tissue. Consequences of the biopsy protocol design on muscle protein quantity and quality rem...

Authors: Marc-André Caron, Steve J Charette, François Maltais and Richard Debigaré

Computational toxicology using the OpenTox application programming interface and Bioclipse

Toxicity is a complex phenomenon involving the potential adverse effect on a range of biological functions. Predicting toxicity involves using a combination of experimental data (endpoints) and computational m...

Authors: Egon L Willighagen, Nina Jeliazkova, Barry Hardy, Roland C Grafström and Ola Spjuth

Mid-esophagus unresectable cancer treated with a low cost stent. First experience

In the cancer of the esophagus, with recent technologic advances, self-expanding metal stents (SEMS) are at the forefront of the armamentarium for re-establishing luminal patency. Weighed against the numerous ...

Authors: Valter N Felix, Alaor Caetano, Jose P Cipullo, Emiliano C Almodova, Wagner Colaiacovo and Aldenir F Zamboti

Pattern of childhood burn injuries and their management outcome at Bugando Medical Centre in Northwestern Tanzania

Burn injuries constitute a major public health problem and are the leading cause of childhood morbidity and mortality worldwide. There is paucity of published data on childhood burn injuries in Tanzania, parti...

Authors: Phillipo L Chalya, Joseph B Mabula, Ramesh M Dass, Geofrey Giiti, Alphonce B Chandika, Emmanuel S Kanumba and Japhet M Gilyoma

Understanding socio-economic determinants of childhood mortality: a retrospective analysis in Uganda

Teso sub-region of Eastern Uganda had superior indices of childhood survival during the period 1959 to 1969 compared to the national average. We analysed the reasons that could explain this situation with a vi...

Authors: Fred Nuwaha, Juliet Babirye, Olico Okui and Natal Ayiga

Comparative analysis of miRNAs and their targets across four plant species

MicroRNA (miRNA) mediated regulation of gene expression has been recognized as a major posttranscriptional regulatory mechanism also in plants. We performed a comparative analysis of miRNAs and their respectiv...

Authors: Dorina Lenz, Patrick May and Dirk Walther

Expedited batch processing and analysis of transposon insertions

With advances in sequencing technology, greater and greater amounts of eukaryotic genome data are becoming available. Often, large portions of these genomes consist of transposable elements, frequently account...

Authors: Jeremy D Smith and David A Ray

Characteristics of patients presenting to the vascular emergency department of a tertiary care hospital: a 2-year study

The structure of health care in Greece is receiving increased attention to improve its cost-effectiveness. We sought to examine the epidemiological characteristics of patients presenting to the vascular emerge...

Authors: Ioannis Kotsikoris, Theofanis T Papas, Nikolaos Papanas, Dimitrios Maras, Paraskevi Tsiantula, Polyvios Pavlidis, Maria Andrikopoulou, Stamatia Kotsiou, Efstratios Maltezos and Nikolaos Bessias

Determining specific competencies for General Internal Medicine residents (PGY 4 and PGY 5). What are they and are programs currently teaching them? A survey of practicing Canadian General Internists

General Internal Medicine (GIM) has recently been approved as a subspecialty by the Royal College of Physicians and Surgeons of Canada. As such, there is a need to define areas of knowledge that a General Inte...

Authors: Sharon E Card, Anne M PausJenssen and Rachel C Ottenbreit

Hepatitis B virus infection among medical waste handlers in Addis Ababa, Ethiopia

Healthcare wastes contain a wide range of microorganisms among which hepatitis B virus (HBV) are the most significant pathogens. No data about the prevalence of HBV among medical waste handlers is available in...

Authors: Yitayal Shiferaw, Tamrat Abebe and Adane Mihret

Ertapenem for treatment of osteomyelitis: a case series

Ertapenem is a once-daily broad spectrum carbapenem that is increasingly used to treat polymicrobial osteomyelitis due to diabetic foot and traumatic wound infections. However, limited data exists on ertapenem...

Authors: Neela D Goswami, Melissa D Johnson and Vivian H Chu

Oxidative stress and leaf senescence

Senescence is an important developmental process that leads to the cell death through highly regulated genetically controlled processes in plants. Biotic and abiotic Oxidative stresses can also artificially in...

Authors: Hatami Gigloo Sedigheh, Mahdi Mortazavian, Dariush Norouzian, Mohammad Atyabi, Azim Akbarzadeh, Keyvan Hasanpoor and Masoud Ghorbani

Mutation screening of patients with Alzheimer disease identifies APP locus duplication in a Swedish patient

Missense mutations in three different genes encoding amyloid-β precursor protein, presenilin 1 and presenilin 2 are recognized to cause familial early-onset Alzheimer disease. Also duplications of the amyloid ...

Authors: Håkan Thonberg, Marie Fallström, Jenny Björkström, Jacqueline Schoumans, Inger Nennesmo and Caroline Graff

Sputum smear negative pulmonary tuberculosis: sensitivity and specificity of diagnostic algorithm

The diagnosis of pulmonary tuberculosis in patients with Human Immunodeficiency Virus (HIV) is complicated by the increased presence of sputum smear negative tuberculosis. Diagnosis of smear negative pulmonary...

Authors: Hedwiga F Swai, Ferdinand M Mugusi and Jessie K Mbwambo

Antiviral mode of action of bovine dialyzable leukocyte extract against human immunodeficiency virus type 1 infection

Bovine dialyzable leukocyte extract (bDLE) is derived from immune leukocytes obtained from bovine spleen. DLE has demonstrated to reduce transcription of Human Immunodeficiency Virus Type 1 (HIV-1) and inactiv...

Authors: Humberto H Lara, Liliana Ixtepan-Turrent, Elsa N Garza-Treviño, Jose I Badillo-Almaraz and Cristina Rodriguez-Padilla

Family physician attitudes in managing obesity: a cross-sectional survey study

Obesity is epidemic in primary care. While family physicians care for the consequences of obesity, they do not generally feel confident managing obesity itself. We examined the barriers to obesity management i...

Authors: John W Epling, Christopher P Morley and Robert Ploutz-Snyder

Intestinal parasitosis and shigellosis among diarrheal patients in Gondar teaching hospital, northwest Ethiopia

Diarrheal diseases are the major causes of morbidity and mortality in developing world. Understanding the etiologic agents of diarrheal diseases and their association with socio-demographic characteristics of ...

Authors: Kahsay Huruy, Afework Kassu, Andargachew Mulu, Netsanet Worku, Teshome Fetene, Simon Gebretsadik, Fantahun Biadglegne, Yeshambel Belyhun, Abebe Muche, Aschalew Gelaw, Belay Anagaw, Sisay Yifru, Yemataw Wondie, Assegedech Bekele, Moges Tiruneh, Dieter Reissig…

Computational analysis of expression of human embryonic stem cell-associated signatures in tumors

The cancer stem cell model has been proposed based on the linkage between human embryonic stem cells and human cancer cells. However, the evidences supporting the cancer stem cell model remain to be collected....

Authors: Xiaosheng Wang

Cyclic stretch increases splicing noise rate in cultured human fibroblasts

Mechanical forces are known to alter the expression of genes, but it has so far not been reported whether they may influence the fidelity of nucleus-based processes. One experimental approach permitting to add...

Authors: Michael Uhl, Kevin Mellert, Britta Striegl, Martin Deibler, Markus Lamla, Joachim P Spatz, Ralf Kemkemer and Dieter Kaufmann

TP53 mutations, human papilloma virus DNA and inflammation markers in esophageal squamous cell carcinoma from the Rift Valley, a high-incidence area in Kenya

Squamous Cell Carcinoma of Esophagus is one of the most common malignancies in both men and women in eastern and south-eastern Africa. In Kenya, clinical observations suggest that this cancer is frequent in th...

Authors: Kirtika Patel, Simeon Mining, Johnston Wakhisi, Tarik Gheit, Massimo Tommasino, Ghislaine Martel-Planche, Pierre Hainaut and Behnoush Abedi-Ardekani

Molecular epidemiology of hepatitis C infection in Cyprus within the general population and high-risk cohorts

Initial data on the molecular epidemiology of HCV infection in Cyprus showed a highly polyphyletic infection and multiple points of introduction into the general population. The continuation and expansion of t...

Authors: Victoria L Demetriou and Leondios G Kostrikis

Clinical application of a rapid microbiological test based on capillary zone electrophoresis to assess local skin infection

The basic clinical problem associated with infection treatment is the fact that classic, commonly and routinely used isolation and identification methods are based on long-term processes of a phenotypic analys...

Authors: Jacek Szeliga, Marek Jackowski, Ewa Kłodzińska, Bogusław Buszewski and Wojciech Kupczyk

Acidochromogenicity is a common characteristic in nontuberculous mycobacteria

An acidic environment is something likely encountered by mycobacteria in the environment or in a human host. Previously mycobacterial species had been known to produce carotenoid pigments in response to light ...

Authors: Beatrice Saviola and Jeffrey Felton

Serologic evidence of human orthopoxvirus infections in Sierra Leone

Orthopoxviruses, including variola virus, vaccinia virus, and monkeypox virus, have previously been documented in humans in West Africa, however, no cases of human orthopoxvirus infection have been reported in...

Authors: Adam MacNeil, Jason Abel, Mary G Reynolds, RR Lash, Richard Fonnie, Lansana D Kanneh, Willie Robert, Victor K Lungay, Augustine Goba, Lina M Moses, Inger K Damon, Kevin Karem and Daniel G Bausch

Aneurismal subarachnoid hemorrhage in a Chilean population, with emphasis on risk factors

Subarachnoid Hemorrhage (SAH) is caused principally by the rupture of intracranial aneurisms. Important risk factors have been described such as age, sex, hypertension (HT) and season of the year, among others...

Authors: Mónica Y Acuña and Lucía A Cifuentes

Kdr-based insecticide resistance in Anopheles gambiae s.s populations in Cameroon: spread of the L1014F and L1014S mutations

The spread of insecticide resistance in the malaria mosquito, Anopheles gambiae is a serious threat for current vector control strategies which rely on the use of insecticides. Two mutations at position 1014 of t...

Authors: Philippe Nwane, Josiane Etang, Mouhamadou Chouaїbou, Jean Claude Toto, Rémy Mimpfoundi and Frédéric Simard

Functional Genomics Assistant (FUGA): a toolbox for the analysis of complex biological networks

Cellular constituents such as proteins, DNA, and RNA form a complex web of interactions that regulate biochemical homeostasis and determine the dynamic cellular response to external stimuli. It follows that de...

Authors: Ignat Drozdov, Christos A Ouzounis, Ajay M Shah and Sophia Tsoka

Efficacy of fixed-dose amlodipine and losartan combination compared with amlodipine monotherapy in stage 2 hypertension: a randomized, double blind, multicenter study

The objective of this trial was to compare the blood-pressure lowering efficacy of amlodipine/losartan combination with amlodipine monotherapy after 6 weeks of treatment in Korean patients with stage 2 hyperte...

Authors: Sung-Hae Kim, Kyu-Hyung Ryu, Nam-Ho Lee, Jin-Ho Kang, Woo-Shik Kim, Sang-Weon Park, Hae-Young Lee, Jae-Joong Kim, Young-Keun Ahn and Soon-Yong Suh

Periodontal status of rheumatoid arthritis patients in khartoum state

Few studies have investigated the periodontal condition among Rheumatoid arthritis in Sudan. The present study described the periodontal condition among Sudanese patients suffering from rheumatoid arthritis an...

Authors: Safa K Abdelsalam, Nada T Hashim, Emitithal M Elsalamabi and Bakri G Gismalla

Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes

Plant defensins are an important component of the innate defence system of plants where they form protective antimicrobial barriers between tissue types of plant organs as well as around seeds. These peptides ...

Authors: Abré de Beer and Melané A Vivier

An observational prospective study of topical acidified nitrite for killing methicillin-resistant Staphylococcus aureus (MRSA) in contaminated wounds

Endogenous nitric oxide (NO) kills bacteria and other organisms as part of the innate immune response. When nitrite is exposed to low pH, NO is generated and has been used as an NO delivery system to treat ski...

Authors: Anthony D Ormerod, Amjad AJ Shah, Hong Li, Nigel B Benjamin, Gail P Ferguson and Carlo Leifert

False negative results from using common PCR reagents

The sensitivity of the PCR reaction makes it ideal for use when identifying potentially novel viral infections in human disease. Unfortunately, this same sensitivity also leaves this popular technique open to ...

Authors: Dean J Bacich, Kathryn M Sobek, Jessica L Cummings, Allison A Atwood and Denise S O'Keefe

Erratum to: Selection of reference genes for qRT-PCR examination of wild populations of Atlantic cod Gadus morhua

Authors: Pal A Olsvik, Liv Softeland and Kai K Lie

Invasion of Ureaplasma diversum in bovine spermatozoids

Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze i...

Authors: Melissa Buzinhani, Maurício Yamaguti, Rosângela C Oliveira, Beatriz A Cortez, Lucas Miranda Marques, Gláucia M Machado-Santelli, Mayra EO Assumpção and Jorge Timenetsky

gViz, a novel tool for the visualization of co-expression networks

The quantity of microarray data available on the Internet has grown dramatically over the past years and now represents millions of Euros worth of underused information. One way to use this data is through co-...

Authors: Raphaël Helaers, Eric Bareke, Bertrand De Meulder, Michael Pierre, Sophie Depiereux, Naji Habra and Eric Depiereux

Exploring the influence of sterilisation and storage on some physicochemical properties of coconut (Cocos nucifera L.) water

Fresh coconut (Cocos nucifera L) water is a clear, sterile, colourless, slightly acidic and naturally flavoured drink, mostly consumed in tropical areas. It is a rich source of nutrients and has been used for med...

Authors: Adolf K Awua, Edna D Doe and Rebecca Agyare

Sensitivity of a national coronial database for monitoring unnatural deaths among ex-prisoners in Australia

The period immediately after release from custody is a time of marked vulnerability and increased risk of death for ex-prisoners. Despite this, there is currently no routine, national system for monitoring ex-...

Authors: Jessica Y Andrews, Simon Forsyth, Jessica Wade and Stuart A Kinner