ClonorESTdb: a comprehensive database for Clonorchis sinensis EST sequences

Background Clonorchiasis, which is primarily caused by liver fluke (Platyhelminthes), is a fatal infectious disease that is mainly associated with bile duct malignancy and the subsequent development of cholangiocarcinoma. Thus, a genomic approach now represents an important step to further our knowledge of biology and the pathology of these parasites. The results of expressed sequence tags (ESTs) sequencing need to be well organized into databases to provide an integrated set of tools and functional information. Findings Here, the ClonorESTdb database represents a collection of Clonorchis sinensis ESTs that is intended as a resource for parasite functional genomics. A total of 55,736 successful EST sequences, which are cleaned and clustered into non-redundant 13,305 C. sinensis assembled EST sequences (6,497 clusters and 6,808 singletons), were obtained from three in-house prepared cDNA libraries of C. sinensis at different developmental stages. The assembled consensus sequences were annotated using the BLAST algorithm or/and hmm against NCBI NR, UniProt, KEGG and InterProScan. The ClonorESTdb database provides functional annotation, their expression profiles, tandem repeats and putative single nucleotide polymorphisms with utility tools such as local BLAST search and text retrieval. Conclusions This resource enables the researcher to identify and compare expression signatures under different biological stages and promotes ongoing parasite drug and vaccine development and biological research. Database URL:http://pathod.cdc.go.kr/clonorestdb/

of thousands of sequencing reads in parallel. The genome and transcriptome sequences of a growing number of model organisms have been published in recent years, which have drawn new insights into parasite research [12,13]. However, the de novo large-scale sequencing of a non-model parasite is still a laborious task and an interesting challenge. Expressed sequence tags (ESTs) are a cost effective alternative and a powerful tool that provides sufficient information about functional proteins. In particular, the ESTs from a full-length cDNA library allow researchers to be cloned and provide material sources so that many intriguing biological issues can be isolated.
The aim of this study is to provide important database resources for the characterization and understanding of the functional genes of C. sinensis. Here, we have constructed and described ClonorESTdb, a web-based ESTs database resource that involves systematic functional annotation, which comprises more than 55,736 high-quality ESTs based on three full-length enriched cDNA libraries. The ESTs obtained were assembled into 13,305 C. sinensis Assembled EST sequences (CsAEs) comprising 6,497 clusters and 6,808 singletons by aligning CsAEs onto the non-redundant public NCBI NR database, UniProt, KEGG, InterProScan and Gene Ontology (GO). The ClonorESTdb database described here provides key insights into the differential gene expression of C. sinensis in a range of developmentally relevant conditions.

Database architecture
The ClonorESTdb database runs on a RedHat Enterprise Linux 5.5 platform with the Apache web server version 2.2. We also used the relational Oracle database 11 g standard version to develop and support an integrated database schema for storing sequence data, preprocessed data and final functional annotation. The web application was implemented with JSP (Java Server Pages), JavaServelet technology and the AJAX framework. The web interfaces were designed using HTML language with some scripts in JavaScript and the pages utilized cascading style sheet (CSS) properties. The database is currently optimized to work best with Microsoft Internet Explorer 8 (optimal resolution 1024 × 800).

Data source
We constructed full-length cDNA libraries (adult, metacercaria and egg) from C. sinensis and generated large-scale 60,768 ESTs data by 5'-end sequencing of individual clones [14]. All of the raw and cleaned data can be downloaded from the ClonorESTdb database.
The pipeline for constructing the database In our study, a total of 55,736 C. sinensis EST sequences that were derived from three cDNA libraries (adult, metacercaria and egg) were used to construct the database.
To analyze the data, we developed a pipeline for the ClonorEST Project divided into three steps: sequence cleaning, sequence clustering and assembly, and automatic annotation.

Sequence cleaning
Cleaning is an important part of processing and is used to obtain high-quality EST datasets from raw EST sequences. After base calling was performed using Phred [15], the cleaning process implemented Cross_match (version 0.990329) for masking any vector and contaminant sequences, SeqClean (http://seqclean.sourceforge.net/) for eliminating undetermined bases, poly(A) tails and low complexity elements, RepeatMasker (http://www. repeatmasker.org/) for removing interspersed repeats, such as SINEs (short interspersed nuclear element), LINEs (long interspersed nuclear elements), LTRs (long terminal repeat) and DNA elements included in the Repbase repetitive element library (http://www.girinst. org/) [16] ( Figure 1A).

Sequence clustering and assembly
The clustering procedure was a basic step that was used to collect overlapping CsAEs sequences that originated from the same transcript of a single gene; this is performed to reduce redundancy. The assembly procedure is executed to align and merge many overlapping EST sequences of a much longer DNA sequence to reconstruct a putative fulllength transcript sequence. For clustering and assembling, we used TGICL to create a grouping EST sequences and CAP3 for assembling the clustered EST sequences [17,18] ( Figure 1B).

Automatic functional annotation
For more accuracy and further variety of functional annotation, we used various annotation algorithms and public databases. First, we assigned putative functions to the CsAEs based on BLASTN (Query Coverage ≥ 80.0%, Identity ≥ 70.0%, E-value ≤ 1.0e-5), BLASTX and TBLASTX (Match No. ≥ 30 aa, Identity ≥ 25.0%, E-value ≤ 1.0e-5) searches against the GenBank NT and NR databases (ftp://ftp.ncbi.nih.gov/blast/db/FASTA/). Second, metabolic pathways are extremely important for correctly inferring pathogen invasion, host defense, adaptation, pathogen life cycle and host-pathogen interactions. To get a rationale for the development of anti-parasitic drugs and vaccines, we must identify all parasite-specific metabolic pathways. To identify the pathways, additional annotations were created against the UniProtKB database (http://www.ebi.ac.uk/ uniprot) and the KEGG database (http://www.genome. jp) using BLASTX (Match No. ≥ 30 aa, Identity ≥ 25.0%, E-value ≤ 1.0e-5). All BLAST algorithms were implemented using a TimeLogic DeCypher system (Active Motif, Inc., http://www.activemotif.com). We also used the InterProScan tool to extract additional functional domains (E-value ≤ 1.0e-4). To gain a better classification of the biological function of the CsAEs, an analysis of the functions was conducted using GO terms according to three categories: molecular function, biological process and cellular component. We used Tandem Repeats Finder (TRF) and AutoSNP to detect structural variations [19,20] ( Figure 1C).

Utility and discussion
The aims of this database project included 1) the construction of an integrative database of the C. sinensis transcriptome, 2) the maintenance of a well-organized functional annotation and the identification and characterization of factors such as parasite specific antigen and 3) data-mining for tissue-specific expression to discover key pathways related to parasite proliferation that are essential to its maintenance. The database presented here and named ClonorESTdb consists of 55,736 EST entries from C. sinensis at three stages including adult, metacercaria and egg.

ClonorESTdb web interface
The ClonorESTdb provides a user-friendly interface with the seven following options in the main menu: (1) a detailed pre-processing report, (2) clustering and assembling report and viewer, (3) various functional annotation report and (4) download all of the raw data and the analyzed result. The data can be viewed at various degrees of detail, either as an overview (a list of search results) or as a detailed results page for a selected sequence and functional annotation.

Pre-processing and assembling report
After pre-processing, all of results are stored in a database for evaluation and reporting, according to four steps (base calling, vector trimming, contamination, trimming and repeat masking). The pre-processing shows a summary table of the pre-processing steps. The assembly report of ClonorESTdb provides a comprehensive summary of the status of contigs and singletons, such as sequencing status and clustering information. Furthermore, each sequence and each contig were assigned a detailed on-the-fly page where the ID of the nucleotide sequence can be clicked. In each contig page, the graphical display of the contigs and the contig alignment sequence information are provided. We also provide the module of a trace viewer that allows users to evaluate the result of the sequencing of raw data that was derived from the machine on the web (Figure 2A).

Annotation report
For various functional annotation reporting, the online database of ClonorESTdb provides a comprehensive Figure 1 Workflow scheme of an EST analytic process. A. The cleaning step is composed of base calling (Phred), vector masking (cross_match), contaminant trimming (SeqClean) and repeat masking (RepeatMasker). B. The cleaned EST sequences are sent to the next step: clustering and assembling (TGICL). C. CsAEs sequences are aligned with various public databases for assigning their putative function and predicting structural variation (AutoSNP and TRF).
information system for analyzing data from the 55,736 EST sequencing project. An annotation report provides 12 categories of analysis information to allow users to easily monitor all of the analyzed EST datasets. The "statistics" page provides a numeric table of functional annotation and the annotated species distribution derived from all the algorithms ( Figure 2B). On the "summary report" page, users can see all of the detailed annotation information regarding how the CsAEs were annotated in the system and the users can download entire annotation files as Excel files ( Figure 2C). The annotation results against various databases contain clone and contig information, including the sequence, putative identification, annotation detailed information, a link to the nearest homologue in the public database, the length of the homologous sequence and the percentage identity of the nearest match. In addition, existing information for all CsAEs in an EST set can be retrieved using full-text matching (against read id, consensus id, gene name, gene accession number, functional description and E-value score) from the search tool ( Figure 2D). In this section, ClonorESTdb provides access to the enzyme and pathway information in UniProt and KEGG linked to an external database. Users can mine the enzyme information from the annotated results of KEGG and UniProt databases that contained an EC number in the description ( Figure 2E). Here, for the task of representing and processing information by domain analysis in the "InterPro" page, their products and their functions are presented in GO categories ( Figure 2F). When working on one or more given individuals or species, the biologist may wish to search for markers in intra-specific polymorphisms or intra-specific polymorphisms. Thus, we also added single nucleotide polymorphisms (SNPs) and simple tandem repeats (STRs) data.

Conclusion
In summary, ClonorESTdb is the first incorporated online transcriptome database of C. sinensis that can be freely accessed and downloaded. Moreover, this database provides various utility functions for similarity searches, transcriptome statistic information and investigation of user-supplied genomics and transcriptome sequences. The increasing amounts of genomics data that are derived from the advent of high-throughput technology will further stimulate the integration of ClonorESTdb. Studies of this project are undoubtedly of notable interest to many biologists given the complex genetic, biochemical, physiological and evolutionary processes that remain at the heart of host-pathogen interactions. In addition, we will continue to collect known C. sinensis full-length cDNAs to update the database for public use. The annotated functional protein presented herein and our knowledge database will be useful for parasite researchers who wish to clone and confirm full-length C. sinensis cDNAs of interest.

Availability
The ClonorESTdb is open and freely available. All questions, comments and requests should be sent via email to todaewon@gmail.com.
Other requirements: none. License: None required.