Clinical outcome and risk factors of neonatal sepsis among neonates in Felege Hiwot referral Hospital, Bahir Dar, Amhara Regional State, North West Ethiopia 2016: a retrospective chart review

Sepsis remains a major cause of morbidity and mortality among neonates. The risk factors and clinical outcomes of sepsis are poorly understood. Most cases of sepsis occurred mostly within the first week of newborns life related to perinatal risk factors. Late onset sepsis is related to hospital acquired infections which is seen after seven days of age. The purpose of this study was to assess clinical outcome and risk factors of neonatal sepsis in Felege Hiwot referral hospital Bahir Dar, North West Ethiopia. Among the total 225 neonatal charts reviewed; 164 (72.9%) were age less than or equal to 7 days, and 144 (64%) were males. About 29 (12.9%) neonates were with irregular respiratory signs and 40 (17.8%) had meconium aspiration syndrome. Regarding the clinical outcome of neonatal sepsis: 189 (84%) were improved after treatment, 9 (4%) were died and 13 (5.8%) referred to other organizations for further treatment. Respiratory distress syndrome [AOR = 0.258 (0.072–0.930)] and meconium aspiration syndrome [AOR = 0.1989 (0.059–0.664)] were the determinant factors for poor outcome of neonatal sepsis. The clinical outcome of neonatal sepsis in Felege Hiwot referral hospital was not satisfactory. The significant risk factors for poor outcome of neonatal sepsis were respiratory distress syndrome and meconium aspiration syndrome. Recommendations to improve neonatal outcome are: performing essential newborn care for all newborns and arranging appropriate follow up until the end of neonatal period, increasing antenatal care and early detection and management of neonatal infections or problems.


Background
Neonatal sepsis is a systemic infection occurring in infants within 28 days of life and is a major cause of morbidity and mortality in newborns [1]. According to the international pediatric consensus conference of 2001, neonatal sepsis was defined as systemic inflammatory response syndrome in the presence of or as a result of suspected or proven infection with or without accompanying bacteremia, documented by a positive blood culture in the first 28 days of life [2].
Sepsis encompasses various systemic infections of the new born such as: septicemia, meningitis, pneumonia, arthritis, osteomyelitis and urinary tract infections [3]. Neonatal sepsis is caused by both gram-positive and gram negative bacteria's [4,5].
Neonatal sepsis is classified into two major categories based on the time of onset: early-onset neonatal sepsis (EONS) and late onset neonatal sepsis (LONS). Earlyonset neonatal sepsis appears within the first seven days of life and most cases appear within 24 h of birth. While late onset neonatal sepsis occurs after 8 days of infants life and is mostly acquired after delivery [5,6].
Sepsis is diagnosed by: a complete white blood cell count with differential, blood culture, urine cultures, and a lumbar puncture for cell count and culture. To clear the diagnosis of early onset sepsis factors that predispose the neonate for sepsis such as maternal infection and prolonged rupture of membranes, and prematurity are also considered [1,6].
Signs and symptoms of infection in neonates are subtle and non-specific, may present with one or more of the following: hypothermia or fever, lethargy, poor cry, refusal to suck, poor perfusion, prolonged capillary refill time, hypotonia, absent neonatal reflexes, bulging fontanel, brady/ tachycardia, respiratory distress, apnea and gasping respiration, hypo/hyperglycemia, and metabolic acidosis [3,6,7].
Risk factors for early onset of sepsis includes premature rupture of membrane (PROM), fever, chorioamnionitis, repeated vaginal examination, meconium stained amniotic fluid, dietary intake of contaminated foods, cervical cerclage, place of birth, prematurity, low birth weight, complicated or instrument-assisted delivery, and low appearance pulse grimace activity respiration (APGAR) scores. Late onset of sepsis acquiring nosocomial infections and invasive procedures during hospital admission [1,6,8].
Antimicrobials used to treat sepsis are combinations and in most units are penicillin (Benzyl penicillin, Ampicillin, or Cloxacillin) together with an aminoglycoside, most commonly Gentamicin and is largely preventable by timely recognition, rational antimicrobial therapy and aggressive supportive care [3,9].
Globally, sepsis is one of the major causes of morbidity and mortality among neonates [4], according to WHO sepsis caused approximately 12% of the 2.9 million neonatal deaths in 2012 [10]. Out these deaths 99% occur in developing countries [11].
Therefore the purpose of this study was to assess clinical outcomes and risk factors of neonatal sepsis in Felege Hiwot referral hospital, Bahir Dar, North West Ethiopia.

Study settings and period
An institution based quantitative retrospective chart review was conducted from April 30 to May 30, 2016 in Felege Hiwot referral hospital. It is located in Amhara regional state, Bahir Dar, Ethiopia. It is 565 km away from Addis Ababa. The hospital was established in April 1963 in collaboration with the Ethiopian people and the German government. The hospital has different departments that provide specialized services in outpatient, inpatient and operation theatre departments. It provides services for approximately for 130,000 populations and has more than 415 beds and gives services for the western part of Amhara region as a Referral hospital. Annually nearly 550 neonates with sepsis were admitted at Felege Hiwot referral hospital. The neonatal intensive care unit has 30 beds and there were five pediatricians and 11 nurses.
The sample size of the study was calculated using single population proportion formula by considering the following assumptions: prevalence (P) = 50%, confidence level (CI) = 95%, margin of error (W) = 5% and by using correction formula since the total population is below 10,000 the final calculated sample size became 225.

Measurement
Data was collected and registered by using structured check list. The check list was prepared by reviewing different literatures done on similar topics. The check list consists of socio demographic information of mother and neonate, maternal and neonatal risk factors, and health service related factors for poor outcome of sepsis. The data were collected by four data collectors and one supervisor and finally submitted to the investigator as scheduled. Before the data collection period data collectors and supervisors were oriented and trained for a day on how to record and collect data.

Operational definitions of the variables
Early onset of sepsis: If sepsis is occurred from birth to 7 days of age. Late onset of sepsis: If sepsis is occurred between 8 and 28 days of age. Good outcome: If neonate is improved after completing the treatment without any complications like: seizure, meningitis, shock, deafness and blindness. Poor outcome: If neonate is not improved after completing the treatment, presented with complications, referred to other health institutions, died and refused against medical treatment.

Maternal related risk factor for neonatal sepsis
More than half of the mothers 124 (55.1%) were multigravida. Majority (95.1%) of mothers received ANC follow up and 9 (4%) mothers had history of urinary tract infection during their pregnancy. About 47 (20.9%) mothers were febrile, 12 (5.3%) mothers were twin delivered, 2 (0.9%) were having history of cervical cerclage and 4 (1.8%) were mothers with history medical problem during pregnancy.

Factors associated with clinical outcome of neonatal sepsis
First variables were tested by using bivariate analysis. Variables which were associated (p < 0.05) in the bivariate analysis were tested in the in the final multivariate analysis to see their significant association with poor outcome of neonatal sepsis. The independent predictor of poor outcome of neonatal sepsis were; respiratory distress syndrome and history of meconium aspiration syndrome. Respiratory distress syndrome was significantly associated with poor outcome of neonatal sepsis. Those neonates with respiratory distress syndrome were 74.2% more likely to develop poor outcome (AOR 0.258: 0.072, 0.930) than neonates without respiratory distress syndrome.  Meconium aspiration syndrome was significantly associated with poor outcome of sepsis. Neonates with meconium aspiration syndrome were 80.2% more likely to develop poor neonatal outcome (AOR 0.198: 0.059, 0.664) than neonates without history of meconium aspiration syndrome (Table 5).

Discussion
Neonatal sepsis is a systemic infection occurring in infants at less than 28 days of life and is an important cause of morbidity and mortality of newborns [1]. It encompasses various systemic infections of the new born such as septicemia, meningitis, pneumonia, arthritis, osteomyelitis and urinary tract infections [3].
Risk factors for early onset of sepsis includes: premature rupture of membrane, fever, chorioamnionitis, repeated vaginal examination, meconium stained amniotic fluid, dietary intake of contaminated foods, cervical cerclage, place of birth, prematurity, low birth weight, complicated or instrument assisted delivery, and low appearance pulse grimace activity respiration (APGAR) scores. Late onset of sepsis acquiring nosocomial infections and invasive procedures during hospital admission [1,6,8].
In this study 84% of neonates had good outcome after treatment. This result comparable with a study done in Debrezeyt, Ethiopia favorable outcome of neonatal sepsis were 74% [8] and in Jimma neonatal death due to infections was 34.3% [15], in other studies done in Ethiopia mortalities due to sepsis accounts 5% a hospital based data [14], in Uganda death rates associated with sepsis was 18.1% [16], in Sudan neonatal mortality due to sepsis was found to be 14.5% [13], Egypt mortality rate of neonatal sepsis were 51% for early onset sepsis and 42.9% for   [18]. In this study respiratory distress syndrome was identified as the determinant factor for poor clinical outcome neonatal sepsis. Neonates with history of respiratory distress syndrome were 74.2% more likely to develop poor neonatal outcome. This result comparable with studies done Uganda [16] in which tachypnea (AOR 1.07: 0.65, 1.77) was the determinant factor for poor outcome of sepsis, and in Sudan [19] where tachypnea results 69.4% for poor outcome of sepsis. This was due to health workers ignorance the syndromes, poor early detection of signs and due to the mothers delay to come in health institution.
Meconium aspiration syndrome history was significantly associated with clinical outcome of sepsis. Neonates with meconium aspiration syndrome history were 80.2% more likely to develop poor outcome. Which is similar with a study in Uganda [16] where neonates with meconium aspiration syndrome were 2.5 times more likely to develop poor outcome than neonates without history of meconium aspiration. This is showed that after