War zone refugia? Establishing a baseline for protected waterbirds in a wildlife refuge flanked by agriculture and militarization

Objectives The welfare of threatened fauna should not be assumed merely because their refuges have been designated with protected status. This is particularly true in geographical areas where social/military events drive an under-reported, but potentially lethal, type of human–wildlife interaction. Waterbirds of Gharana Wetland Conservation Reserve consist mostly of threatened species. However, as occurs globally, ‘protected’ fauna near contested borders are sometimes affected by military forces. As part of a larger project to document regional avifauna, we report the seasonal status of waterbirds in order to help establish a baseline for comparing conservation of wildlife within contested areas to that of fauna in more secure refuges. We examined 24 avifauna surveys for relationships between seasons, temperature, individuals and species. Results 28 of 61 waterbird species were rare. We found seasonal variations in individuals (F3,731 = 3.82; P < 0.01) and species (F3,11 = 5.81; P < 0.05) with a major influx in late winter, rather than autumn. Thus, while this sanctuary serves as an over-wintering site, it is also a stop-over site for high-altitude migrations. While providing this baseline, we offer a reminder that the welfare of wildlife in protected areas should be monitored seasonally, with the ongoing threats to their conservation, carefully documented.


Introduction
Human militarization can influence the behaviors and population status of wildlife [1,2] however, few studies documenting this type of human-wildlife conflict (HWC) focus on protected refuges or wildlife sanctuaries [3]. Indeed, gaining the formal protection of a wildlife sanctuary is often considered an end unto itself [4]. The Gharana Wetland Conservation Reserve (GWCR; 32°32′28″N; 74°41′27″E; 281 m asl) is a critical wetland (~ 0.75 km 2 ) situated along the international Indo-Pakistan border. While wetlands provide shelter for more than 12% of all animal species and 40% of all birds [5], this wetland may be especially important because it is found along the Central Asian flyway (Palearctic-Oriental) for winter and summer migrations [6]. The relatively mild winters and abundant resources attract rare and threatened species from as far away as Siberia and Mongolia. However, as occurs in regions throughout the worlddespite the international and local designations for protection [7,8]-the local political and economic conditions challenge the "protected status" of endangered and threatened wildlife.
The principal threats to conservation in GWCR relate directly to its proximity to a contested geopolitical border. Following independence in 1947, both India and Pakistan claimed the same 'line-of-control' [9]. Thus, for the past 70 years, there has been varying levels of military activity, including use of 82 mm mortars, in the area [9]. These shells can influence the wildlife directly through ordinance-strikes, or indirectly through reverberation [10]. In 2003, a formal ceasefire was declared. Ironically, this action increased anthropogenic pressure and revealed a complex dynamic between militarization and agriculture, found here [10], and other regions around the world [11]. Whereas, the declaration of a ceasefire did not completely halt the shelling, but instead limited the threat enough so that farmers moved into cultivate the wetland for farming.

Complex dynamics between militarization and agriculture
Locally, farmers not only endure stray firing, they also compete with wildlife for crops. As occurs in most avifauna refuges, migrant visitors are herbivores, and thus consume seeds, saplings, wheat and even crops such as basmati rice, a local mainstay. When migrants arrive in winter, palatable shoots of wheat and rice seedlings are already germinating [12], Consequently, waterbirds are not only threatened by shelling, but also by farmers who chase them with firecrackers which (ironically, if not purposefully) mimic gunfire and reverberation [13].
Additionally the refuge is compromised because villagers deposit their wastes into the wetland, thus, exacerbating silting [13]. Pollution from fertilizers and domestic animal excreta are further threats. Lastly, some farmers intentionally dump soil into the wetland to increase farmable surface area. The take home lesson is that there is a complex and under-reported dynamic between military and agricultural conflicts. Unfortunately, wildlife in such protected habitats may not be as sheltered as we suppose [14].
From a global perspective, decreases in the abundance or fluctuations of waterbirds are particularly important to document in areas such as these [15]. The conditions of which, may be exacerbated by human presence and 'both types' of disturbance. As such, the status of water-birds-in particular any changes in the number of seasonal migrants-is one of the key distinguishing attributes of its biodiversity when threatened by anthropogenic factors [15]. By formally documenting this information, researchers may help promote the potential for ecotourism, as well as compensation for farmers [16].

Objectives
Contested areas such as GWCR are recognized as "war zone refugia" [3], but fauna within are not well-documented. Our objectives were to seasonally document the residential status, relative abundance, richness, evenness and feeding guilds of waterbirds in the GWCR over a full year. This information represents an essential step in a comprehensive plan to document the wildlife in this protected region, and to obtain baseline data for longitudinal comparisons of secure sanctuaries.

Main text
Methods Gharana 32°32′28″N; 74°41′27″E; 281 m above sea level, located on the Indo-Pakistan border in the south-western part of Jammu and Kashmir, is composed of a rain-fed swamp with a bottom surface of loamy clay with decaying vegetation. It is in the subtropical climatic zone where summer temperatures reach 46 °C maximum and winter minima decrease to 2 °C. Annual rainfall is ~ 1331 mm, with the majority of precipitation occurring when the south-western monsoon winds arrive (July-September). Vegetation includes Eicchornia spp., Hydrilla spp. [17] and the common reed (Typha spp.). Due to local development, there is also surface runoff from agricultural fields [18].
The agricultural fields adjacent to Gharana village also provides both suitable habitat, and concomitant threats, for a diverse group of bird taxa [19]. These characteristics make this protected area both accessible and economically important. This wetland is also located in a state known for outdoor activities and adventure (Jammu and Kashmir), and is internationally renowned for birdwatching and mountaineering [16].

Data collection
Our methods have been previously reported in the overarching project [19], except herein we report seasonal fluctuations limited to waterbirds, as the data was far too prodigious to include in one report. Twenty-four surveys were conducted from July 2012 to June 2013, covering all seasons; summer (April-June), monsoon (July-Sept), autumn (Oct-Novem) and winter (Dec-March). These surveys strictly followed well-established methods for line transects and point count methods in [20] (e.g., using widely-spaced, randomly-elected transects, with attention to avoiding bias from effort, walking speed, or weather conditions; birds flying overhead were counted separately as they cannot be used in density estimation). Counts were performed twice per month at all sites by a team of ten individuals in the early morning (07:00-10:00) during the time of highest bird activity [21] and lowest human disturbance. Observer effects were minimal because these animals have habituated to humans through agricultural and military actions. Experts > 200 h of wetland bird identification and post-doctoral training were consulted throughout the period.
All waterbird species were classified as common/ rare, and also resident/migratory status of the birds as per [22]. For instance, VC = very common species encountered during (80% of all surveys); C = common species encountered frequently (50-70%) and R = rare species which are encountered less frequently (10-20%).
Likewise, if a particular species was documented between December and March, then it was considered as a winter visitor. Whereas, presence between April and June was documented as a summer visitation. If we documented a waterbird was documented throughout a year in and around GWCR, then it was considered as a resident. Feeding guilds were identified from the literature, rather than what birds were seen feeding on at the time. Nikon Monarch 10 × 42 binoculars were used during surveys for taking observations and on-the-spot identification. Photographs and/or video were used to validate any unidentified species. The checklist was prepared using the standardized common and scientific names assigned in [23]. All data collected were observational and did not involve any manipulation or alteration of any animals, plants or humans.

Statistics
Univariate analysis of variance (ANOVA) was used to examine the relationships between season and between number of individuals and the number of species. Tukey's post hoc test was used to test pair-wise comparisons between seasons. Univariate ANOVA was also used to examine the relationship between season and each of four indices (Shannon-Wiener, Simpson's Diversity, Equitability J and the Margalef index). Cross tabulations with Pearson's Chi Square tests were performed between feeding guild and abundance, feeding guild and residence status, and between abundance and residence status. We used linear regressions to assess the relationship individuals and temperature and between species and temperature. Statistical significance (alpha) was set at P ≤ 0.05 and descriptive and inferential analyses were conducted using Minitab V. 17 (State College, PA).

Results
We documented 61 waterbird species from 11 families of 6 orders over 1 year (Table 1); 28 species were rare. The majority were from three families, the Anatidae (Anseriformes), Phalacrocoracida (Pelicaniformes), and Rallidae (Gruiformes). We found the most waterbird species in March (39), and fewest in June (16). The largest population (~ 9701 individuals) was also recorded in March while the lowest population size (130 individuals) was found in May. Order Anseriformes contributed the most species (19). During March, the Bar-headed Goose (Anser indicus) constituted 62% (6000 individuals) while the Ruddy Shelduck (Tadorna ferruginea) accounted for 15% (1500) of the total population (9701) count.
The number of waterbird individuals (ANOVA F 3,731 = 3.82; P < 0.01) and species (ANOVA F 3,11 = 5.81; P < 0.05) varied by season (Table 2). Tukey's post hoc test showed that the number of individuals and species in the Winter differed to all three seasons, and that Autumn, Monsoon, and Summer seasons were not different to one another. Among the 4 indices, the Shannon-Wiener (ANOVA F 3,11 = 25.2; P < 0.001), Simpson's Diversity ANOVA F 3,11 = 18.5; P < 0.001, and Equitability J (ANOVA F 3,11 = 18.6; P < 0.001) were each significantly different across seasons. In all cases winter was different to the other three seasons. The Margalef index (ANOVA F 3,11 = 0.75; P > 0.5) did not vary significantly by season.

Discussion
Protected fauna inhabiting "war zone refugia" have not been well documented [3], despite their presence and vulnerability in geographically-contested areas worldwide [3]. Here, we have provided a template for obtaining baseline data for waterbirds living in such areas. In doing so, we have provided the first documentation of the seasonal status, relative abundance, species richness, evenness and dominance of waterbirds under-duress over 1 year. We have identified 61 species from 11 families of 6 orders; two-thirds of all species (40) were visitors, and almost half (28) were rare. Waterbirds present during the stopover period (March high-altitude return migration) contributed more to the four indices than over-wintering birds (late Autumn upsurge).
Like most wetlands, this reserve supports birds of a diverse array of ecological niches and therefore, varied diets. The majority of resident species were carnivores, likely owing to the wide availability of year-round access to invertebrate fauna. Most of the carnivores were very common, whereas there were no resident herbivores. However, migrants were more likely to be herbivores, partly explaining many locals' frustration with the loss of crops. Furthermore, most of the herbivores visited Continued documentation of the avian fauna and their availability of resources is necessary to aid in the promotion of the wetland for improved conservation. While important for baseline data and continual monitoring, this information may also be utilized to quantify numbers to inspire ecotourism and similar approaches to enhance the livelihood of resident farmers and provide

Limitations
Our data are descriptive, consider ad hoc hypotheses, and do not include comparative data from other wildlife refuges or sanctuaries around the globe. Further, we do not report quantifiable measures of shelling (e.g., number of explosions, amplitude of noise generated, lethality, damage to young, or variation by season). However, we hope our initial communication encourages others to analyse and report the well-being of fauna in both secure and vulnerable sanctuaries in the presence and absence of militarization.