Dyslipidemia and associated factors among women using hormonal contraceptives in Harar town, Eastern Ethiopia

Objective Dyslipidemia is abnormal amount of lipid in blood. Hormonal contraceptives affect lipid metabolism and can enhance the risk of vascular disease like atherosclerosis. In Harar, among contraceptive users, biochemical changes follow up is almost none and magnitude of dyslipidemia is not known. Therefore this study is designed to determine prevalence of dyslipidemia and its predisposing factors. Accordingly, cross-sectional study was conducted from April to June 2014 among hormonal contraceptive users from three health centers and one hospital. Socio-demographic data, anthropometric measurements, and blood biochemical tests were performed for every participant. Descriptive statistics and logistic regression analysis with 95% confidence interval using SPSS was used. Result Totally 365 participants were included and the prevalence of dyslipidemia was 34.8%. The mean levels ± standard deviation of total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), the total cholesterol to HDL ratio, and triglyceride were 186 ± 27 mg/dl, 121 ± 31 mg/dl, 45.21 ± 7.7 mg/dl, 4.44, and 108 ± 3.45 mg/dl, respectively. Age, fasting blood sugar, drinking coffee twice and eating no vegetables 4 times/week were identified as predictors of dyslipidemia. In conclusion, hormonal contraceptive users of Harar have high rate of dyslipidemia. This result emphasizes the urgent need for a public health strategy for prevention, early detection, and treatment of dyslipidemia. Electronic supplementary material The online version of this article (10.1186/s13104-019-4148-9) contains supplementary material, which is available to authorized users.


Introduction
Dyslipidemia is a condition that occurs because of abnormalities in the plasma lipids such as elevated plasma total cholesterol (TC), elevated low-density lipoprotein cholesterol (LDL-C), elevated triglycerides (TG) and reduced high-density lipoprotein cholesterol (HDL-C) levels, occurring singly or in combinations [1]. Together with other cardiovascular risk factors dyslipidemia may influence the progression of atherosclerosis [2]. Cardiovascular disease (CVD) is the leading cause of death in women and the third leading cause of death among women of reproductive age. During 2005 through 2008, 11% of women aged 20 to 44 years had dyslipidemia [3,4]. Hormonal contraceptives affect cardiac function, blood pressure (BP), fat and carbohydrate metabolism [5]. The resultant effect of contraceptive hormones on lipid metabolism depends on the type and dose of the compounds, the route of administration, and the duration of treatment [6]. Prolonged use of hormonal contraceptives by females during their reproductive age can induce metabolic changes that may contribute to an increased risk of coronary heart [7]. It is now generally accepted that both elevated levels of non-HDL-C and low HDL-C concentrations may promote the development of atherosclerosis [8]. As the resultant effect of combined oral contraceptives (COC) on the formation of dyslipidemia depends on different factors and dyslipidemia promotes development of atherosclerosis, determining magnitude of dyslipidemia and other cardiovascular risk factors among Open Access BMC Research Notes *Correspondence: waqtolachalt@yahoo.com 2 School of Medical Laboratory Sciences, Faculty of Health Sciences, Institute of Health, Jimma University, Jimma, Ethiopia Full list of author information is available at the end of the article women using hormonal contraceptive helps to aware health care workers to use options for its management. However, there is no study for determination of dyslipidemia and cardiovascular risk factors for women using hormonal contraceptives in all health facilities in Harar town. Hence, the objective of this study is to determine the prevalence of dyslipidemia and associated factor among women using hormonal contraceptives.

Study setting and participants
A cross sectional study was conducted from April to June, 2014 in Harar Eastern Ethiopia, which was selected randomly and is 515 km away from Addis Ababa. Sample size was determined using single population proportion formula and then corrected by finite population correction formula to include a total of 365 hormonal contraceptive users having complete recorded data, satisfied the inclusion criteria and volunteer to participate in the study. To include participants randomly from the town, we stratified the town into three areas and randomly selected at least 1 public health institution from every area. Participants were included consecutively from 3 health facilities (2 health centers and 1 hospital) in the town till the determined sample size was achieved. The 3 facilities were selected randomly. The 365 sample size was divided proportionately among the 2 health centers and the 1 hospital as 1:1:1.65 ratios, respectively. Hormonal contraceptive users with the current history of pregnancy; those who have history of hypertension, diabetes, HIV/AIDS, mental disorders and those who were on lipid lowering medications were excluded from the study.

Data collection and laboratory testing
Socio demographic and clinical data were collected using structured questionnaire and checklist. Each consented participants were informed about the importance of obtaining overnight fasting blood specimen and advised to come early in the morning by the next day; then about 10 ml of venous blood was collected from each into clean serum separator test tubes and allowed to clot for 30 min. After retracting the clot the samples were centrifuged at 3000 rpm's for 10 min and 5 ml pure serum samples were transferred to nunc tubes and analyzed for fasting blood sugar (FBS), TC, TG, and LDL-C using Mindray BS-120 chemistry analyzer. HDL-C was calculated in serum specimens having a TG value < 400 mg/dl by reverse method using Friedewald formula.
Standard operating procedures and manufacturer instructions were strictly followed throughout the procedures. Quality control run was undertaken for all laboratory tests in this study.

Statistical analysis
Data was edited, cleaned, coded and entered using Epidata and then exported to SPSS for analysis. Mean, standard deviation and frequency of quantitative variables were calculated. Bivariate and multivariate logistic regression analysis was calculated with 95% confidence interval (CI) to evaluate the possible association of the variables and p-value of less than 0.05 was considered as statistically significant.

Demographic and anthropometric data
A total of 365 consented women were enrolled in this study with overall response rate of 100%. The mean plus or minus standard deviation (± SD) age of the participants was 30.1 (± 6.7) years. Majority, 163 (44.7%), of the participants were 20-29 years old. The mean height and weight of the subjects were 161 (± 0.06) cm and 57.9 (± 6.8) kg, respectively. Majority, 187 (51.2%), of the participants were Muslim by religion and Oromo by ethnicity [162 (44.4%)]. Educationally, majority of them, 206 (56.4%), attended above secondary school; occupationally 157 (43%) of them were housewives and 307 (84.1%) were married. The mean monthly income of the family was 1786.6 Ethiopian birr (ETB) ( Table 1).

Clinical and laboratory data
The mean (± SD) levels of TC, LDL-C, HDL-C, TG, and the TC/HDL-C ratio of the study subjects were 186 (± 27) mg/dl, 121 (± 31) mg/dl, 45.21 (± 7.7) mg/dl, 108 (± 3.45) mg/dl, and 4.44, respectively. The age-adjusted mean (± SD) levels of the lipid profiles are presented in tables (Additional file 1: Table S1). Moreover their mean (± SD) in comparison with types and duration of contraceptives used are presented in tables (Additional file 2: Table S2 and Additional file 3: Table S3).

Prevalence of dyslipidemia
Dyslipidemia or abnormal values of TC, TG, LDL, HDL and TC/HDL ratio were identified, with overall prevalence of 34.8% (Additional file 4: Figure S1). Sociodemographic variables such as age, occupation, income, religion, marital status and educational status were taken as study variables. The presence of dyslipidemia was statistically different among age categories. However, there was no difference among religion, ethnicity, marital status, educational status and occupation (Table 1). Additionally, the presence of dyslipidemia was assessed based on BP, BMI, waist circumference, waist-to-hip ratio (WHR), FBS, life style, feeding habits, family history of selected medical condition, parity and type and duration of hormonal contraceptives. Women with dyslipidemia were 10 times likely to have SBP > 140 mmHg (Odds ratio (OR) = 10.085; 95% CI 2.175-46.78, p = 0.003) and 8 times likely to have DBP > 90 mmHg (OR = 7.695; 95% CI 2.78-21.27, p = 0.000) as compared to their counterparts. However, there was no difference in dyslipidemia between types of contraceptives, drinking alcohol and cigarette smoking ( Table 2). The proportion of women having dyslipidemia in comparison with the type and duration of use of contraceptive are presented in table (Additional file 5: Table S4). The percentage of dyslipidemia in respect to individual biochemical parameters of the lipid profiles are displayed in table (Additional file 6: Table S5).

Predictors of dyslipidemia
The significant predictors of dyslipidemia in this study were age, FBS, not eating vegetables and drinking coffee twice. Women who do not eat vegetables were about 3 times more likely to develop dyslipidemia (adjusted odds ratio (AOR) = 3.14; 95% CI 1.27-7.77, p = 0.013) and those who drink coffee more than 2 times per day were about 5 times more likely to develop dyslipidemia (AOR = 4.810; 95% CI 1.23-18.76, p = 0.024) ( Table 3).

Discussion
Dyslipidemia is a common public health problem in developing countries, the prevalence of which is rising  steadily [9]. To the best of our knowledge this study is the first to examine prevalence of dyslipidemia and associated factors in Ethiopia. In this study the prevalence of dyslipidemia was found to be 34.8% which is lower than finding in Iran (63.4%) [9]. The predictors of dyslipidemia were age > 30 years, FBS > 110 mg/dl, coffee drinking > 2 times a day and not eating vegetables. The result for not eating vegetables agrees with the study conducted in Framingham [10] but literature on the effect of coffee on serum lipids in women using hormonal contraceptive is limited.
The mean levels of lipid profile are different from finding in Iraq [11]. This difference might be due to the socio-demographic and socioeconomic status of the study subjects.
The prevalence of hypercholesterolemia (TC ≥ 200 mg/dl) was 33.7% and the mean TC level (186 ± 27 mg/dl) of this study is comparable with that in Brazil [12] and Germany [13] but lower than Bangladesh, Dhaka [14] and higher than Iraq [11]. Significantly increased TC might be due to differences in socioeconomic, life style, methodology and duration and type of contraceptives.
The prevalence of hyperlipidemia (LDL-C ≥ 130 mg/dl) is found to be 34.8% and the mean LDL-C (121 ± 31 mg/ dl) is lower than study in Bangladesh, Dhaka [14], but higher than Brazil [12], Germany [13] and Iraq [11]. This variation might be due to differential distribution in risk factors.
The prevalence of hypolipidemia (HDL-C < 40 mg/ dl) was 28.2%. The alteration in the reference value, as to the new recommendations [15] made comparison difficult with the previous studies. The mean HDL-C (45.21 ± 7.7 mg/dl) is lower compared with study in Brazil [12], Germany [13] and Iraq [11] but higher than Bangladesh, Dhaka [14].
The prevalence of hypertriglyceridemia was found to be 17.0% which is comparable with studies in developing countries including Africa where its level ranges from 15 to 30% [16]. The mean TG (107 ± 34 mg/dl) is comparable with finding in Bangladesh, Dhaka [14] but, lower than Basra, Iraq [11] and higher than Pakistan [7]. The decreased prevalence of hypertriglyceridemia in this study might be related to hormonal contraceptive induced increase in clearance of TG rather than synthesis and might be because of fasting.
The mean SBP (119.85 ± 9.5 mmHg) and DBP (77.96 ± 6.8 mmHg) are consistent with the study in Germany [13] but SBP is different from Basra, Iraq [11]. This variation might be due to difference of socio-demographic factors, genetic predisposition, dietary factors, and lack of physical activity.
The level of TC, TG and LDL-C has increased with duration of contraceptive intake but, HDL-C level is significantly decreased. This agrees with a study in Nigeria [17] but the LDL-C level is different from Basra; Iraq [11] in which it was decreased with duration of contraceptive use. Significant decrease (p < 0.05) of HDL-cholesterol was observed within the duration of different types of hormonal contraceptives [18][19][20][21].
Dyslipidemia was higher among groups with high educational level. This might be resulted from higher income, poor working conditions and poor nutritional habits.
The prevalence of dyslipidemia was significantly higher among employed 53 (38.4%) and house wives 52 (33.1%) which might be due to poor physical activity. The odds ratio for dyslipidemia is significantly greater in subjects with a family history of obesity, diabetes and hypertension. Cigarette smoking has no significant association with dyslipidemia which is similar with study conducted in Germany [13].
The prevalence of dyslipidemia has increased with parity, however; there was a fluctuating pattern in HDL-C level in between consecutive pregnancies which is similar to studies according to Mankuta et al. [21].

Conclusion
This study revealed that dyslipidemia is high among the study subjects and age, FBS, drinking coffee twice and not eating vegetables were found to be the independent predictors of dyslipidemia. The finding of this study indicates the urgent need for public health strategy to prevent, detect, and treat the dyslipidemia. Public health measures should continue to emphasize on the importance of healthy feeding habits and screening for different metabolic disorders as well as provision and monitoring of the quality of family planning services. Moreover large scale longitudinal study is needed to determine the effect of hormonal contraceptives on serum lipid and lipoprotein levels. Thus, this study can be used as baseline information for further studies related to effect of hormonal contraceptives on serum lipid levels.