Low food and nutrition literacy (FNLIT): a barrier to dietary diversity and nutrient adequacy in school age children

Objective This study aims to assess the relationship between Food and Nutrition Literacy (FNLIT) and dietary diversity score (DDS); FNLIT and nutrient adequacy (NAR%, MAR%) in school-age children in Iran. Results This cross-sectional study was undertaken on 803 primary school students in Tehran, Iran. Socio-economic, as well as three 24-h dietary recalls were collected through interviewing students and their mothers/caregivers. FNLIT was measured by a self-administered locally designed and validated questionnaire. Low level of FFNL was significantly associated with higher odds of low DDS (OR = 2.19, 95% CI 1.32–3.62), the first tertile of fruit diversity score (OR = 3.88, 95% CI 2.14–6.99), and the first tertile of dairy diversity score (OR = 9.60, 95% CI 2.07–44.58). Low level of IFNL was significantly associated with probability of lower meat diversity score (OR = 1.73, 95% CI 1.07–2.81). Low level of FLL was also significantly associated with probability of lower DDS (OR = 1.81, 95% CI 1.11–2.94), dairy diversity score (OR = 2.01, 95% CI 1.02–3.98), and meat diversity score (OR = 2.14, 95% CI 1.32–3.45).Low FNLIT and its subscales were associated with higher odds of low level of NAR of protein, calcium, vitamin B3, B6, B9, as well as the probability of lower level of MAR.


Introduction
Prevalence of Non-Communicable Diseases (NCDs) is a public health problem in Iran [1]. Therefore, an important priority for the health sector is capacity building within the public to prevent NCDs by empowering people to take control of the determinants of their health and disease [2]. Health literacy is considered as one of the most important skills to enable individuals to control health determinants [3]. Due to wide scope of health issues and because of the growing prevalence of diet-related chronic diseases [4], evidence suggest that one should consider health literacy more specifically [5]. As a result, food literacy/nutrition literacy has been proposed and conceptualized.
Studies have found that food literacy/nutrition literacy can have a critical role in shaping children's dietary behaviors [6] and enabling them to have healthy food choices [7,8]. In Iran, nutrition transition has taken place due to urbanization and rapid socio-economic changes and have resulted in a tendency toward a more westernized dietary pattern, especially among children and adolescents [9]. This general shift in children's diet is characterized as low consumption of fruit and vegetables, fiber-rich foods and dairy products [10], as well as high consumption of fatty, sugary and convenience foods [11]. Considering today's children food environment, improving food and nutrition literacy provides an opportunity for them to acquire appropriate skills and act more consciously [12]. Identifying the relation between food and nutrition literacy and children dietary intake is important for the development of effective prevention and management strategies in these age groups [6]. Yet, there is a gap of published evidence in this area. Therefore, This study aims to assess the relationship between Food and Nutrition Literacy (FNLIT) and dietary diversity score (DDS); as well as FNLIT and nutrient adequacy (NAR%, MAR%) in school-age children in Tehran, Iran.

Study design and setting
This school-based cross-sectional survey was performed using a multistage random cluster sampling design. The study was conducted in Tehran the capital city of Iran from November to January 2016. The STROBE (Strengthening The Reporting of Observational Studies in Epidemiology) study conduct [13] and participant flow is outlined in (Additional file 1: Fig. S1).

Study participants
The sample included 803 primary school students (419 boys and 384 girls) aged 10-12 years (power study 88%; response rate = 89.2%) from different socio-economic districts. Selected students and their parents were invited to take part in the study after signing a consent letter.

Data collection
Food and nutrition literacy assessments Food and nutrition literacy (FNLIT) was measured by a valid self-administered questionnaire [14]. The questionnaire consisted of 4 true-false and 42 likert-type items within two cognitive and skill domains. Cognitive domain included two sub-scales: understanding food and nutrition information (UFNI, 10 items) and nutritional health knowledge (NHK, 5 items). Skill domain consisted of four sub-scales: functional food and nutrition literacy (FFNL, 10 items), interactive food and nutrition literacy (IFNL, 7 items), food choice literacy (FCL, 6 items) and critical food and nutrition literacy (CFNL, 4 items). Finally, Food label literacy (FLL) was evaluated by 4 true-false items. According to ROC analysis, three levels for FNLIT was low (≤ 51), medium (> 51-< 74) and high (≥ 74), where the FNLIT score ranged from 25.8 to 96.8 [15]. The questionnaire was completed by students.
Dietary intake assessments Three 24-h dietary recalls (2 week-days and one holiday) was collected by interviewing the students and their mothers and/or other caregiv-ers. To identify misreporting, BMR (basal metabolic rate) was estimated by using the equations published by Schofield (16). An individual's daily food intake was considered under-reported, if EI (energy intake) to BMR ratio (EI/ BMR) was less than 1.14, or over-reported if it was higher than 2.5 (17). Over-and under-reporters were excluded from the study as 'misreporters' . After data cleaning and excluding all outliers and diet recall misreporters (170 students), 493 students remained for statistical analysis (see Additional file 1: Fig.S1). The characteristics of subjects excluded did not differ significantly from those remained in the study. Dietary intake adequacy-The nutrient adequacy ratio (NAR%)-was calculated for energy and 11 nutrients. NAR was calculated as the intake of a nutrient divided by the recommended intake for that nutrient (RNI) [16]. Mean adequacy ratio (MAR %) was calculated as a measure of the adequacy of overall diet, where MAR is the sum of each NAR (truncated at 100%) divided by the number of nutrients (excluding energy and protein) [17].
Dietary Diversity Score (DDS)-DDS was calculated as part of the pyramid serving database that was categorized into 23 broad food groups. Each of the 5 broad food categories received a maximum diversity score of 2 of the 10 possible score points. To be counted as a "consumer" for any of the food groups categories, a respondent needed to consume at least one-third serving at any time during a 3-day survey period [18].

Covariates
A number of evidence-based covariates [19][20][21][22] were considered in the study. Anthropometric measurements were taken [23]. BMI-Z-score for age and sex was calculated by WHO AnthroPlus, 2007 [24]. Physical activity was measured through interviewing children by the locally validated version of Child and Adolescent International physical activity questionnaires [25,26]. Household food security status was measured using a locally validated 18-item USDA's Household Food Security Survey Module through face-to-face interview with mothers [27,28]. Demographic and socio-economic characteristics were collected by a questionnaire through interviewing with students and verified by their mothers and/or caregivers thereafter.

Statistical analysis
Data were presented as means and standard deviations for continuous variables and frequencies and percentages for categorical variables. Chi square test was used for analysis of general characteristics of participants. Independent sample t test was used to evaluate the differences between continuous variable between two sexes. Multinomial logistic regression analysis adjusting for confounding factors of the lower two tertiles compared with the higher tertile of MAR, NAR and DDS was conducted. All statistical analysis was performed using SPSS 21.0 (SPSS Inc., Chicago, Illinois, US).

Food and nutrition literacy (FNLIT)
FNLIT status of the participants is presented in Fig. 1

Dietary intake adequacy
MAR and NAR of certain nutrients by sex are presented in (Additional file 2: Fig.S2). The multinomial-adjusted odds ratio (95% CI) of the lower two tertiles compared with the highest tertile of MAR and NAR of certain nutrients are presented in (Additional file 3: Table S1).

Dietary diversity
Unadjusted and adjusted multinomial logistic regression of the lower two tertiles compared with the higher tertile of DDS are presented in Table 2. Adjusting for mother education, slightly improved the odds of certain DDS groups, including DDS, fruit diversity score and dairy diversity score for FFNL, and protein foods diversity score for IFNL.

Discussion
The present study showed that low food and nutrition literacy may be a barrier to dietary diversity and nutrient adequacy in school age children. Previous research has shown that high food literacy is associated with increased consumption of fruits and vegetables [29,30]. Children and adolescents who assisted in preparing meals were more likely to engage in food preparation-related behaviors such as buying fresh vegetables, writing grocery lists and preparing meals with chicken, fish or vegetables [31]. Besides, FNLIT skills encompass the ability to obtain factual dietary information and develop an understanding of factors that can enhance or inhibit nutritional health [5] and may promote diet diversity and nutrient adequacy through improving understanding of available food and nutrition information and adherence to the dietary guidelines [5]. Low level of FNLIT was significantly associated with probability of lower level of meat group diversity (as major sources of protein and iron) and NAR of calcium, the two major limiting nutrients in the Iranian's diet [32]. FLL was one of the weakest areas among the studied children. However, the question is to what degree food labeling is an appropriate approach and what type of information and labeling approaches can be helpful for children. Previous reports have identified food label reading as one of the key areas to improve food choices and dietary intakes in children [33]. In Iran, mandatory nutrition labeling for food products has recently been initiated while there are still gaps in its regulatory policy [34]. Besides, this concept and its application has not been entered in public education programs, including schools curricula and textbooks to empower individuals in making healthy food choices [35]. This may explain low scores observed in the study population with regard to questions on food labels.
The considerable proportions of students with low food and nutrition literacy in skill domain imply a gap in food and nutrition skill development in primary school curriculums in the country. This is not a surprise as based on content analysis of primary school textbooks in Iran, nutritional content of school textbooks were mainly theoretical and impractical [35]. This leads to many students  with high food and nutrition knowledge, but limited skills in their dietary practices. Even though, nutrition knowledge has been identified as essential for behavior change [36], knowledge alone is generally not sufficient to produce sustained behavior change in complex behaviors [36]. Therefore, FNLIT subscale-based interventions should be designed to improve students' skills and nutrition behaviors. For example, the results provide a reminder of the need to support improvements in food choices and dietary behaviors by helping young people to develop skills such as those required to interpret food labeling.

Conclusion
This study demonstrates that low level of FNLIT is associated with nutritional inadequacy in school-age childeren and low DDS and may play an important role in shaping their dietary intake. FNLIT is expected to have effect on one's ability to assess information when choosing foods, comprehend food labels, and apply dietary recommendations. Therefore, it is important for educators and program planners to assess and enhance FNLIT of young people. Stakeholders, including policy makers, food manufacturers, health providers, educators, and businesses should also play their roles so as to achieve a bigger impact on future generation.

Limitation
The current study has several limitations.
• First, there was a possibility of recall bias and social desirability bias. • Second, due to its cross-sectional design, our study was not able to establish any cause-effect relationship of food and nutrition literacy on children's dietary intakes. • Third, the excluded data was quite large, due to lack of access to parents by phone, although we found no significant difference in characteristics of dropout subjects with those remained in the study.