Telemetric left ventricular monitoring using wireless telemetry in the rabbit model
- Mallory K Tate†1, 2,
- William S Lawrence†1, 3Email author,
- Randy L Gourley1, 2,
- Diana L Zavala2,
- Lori E Weaver2,
- Scott T Moen1, 3 and
- Johnny W Peterson1, 3
© Lawrence et al; licensee BioMed Central Ltd. 2011
Received: 26 October 2010
Accepted: 5 September 2011
Published: 5 September 2011
Heart failure is a critical condition that affects many people and often results from left ventricular dysfunction. Numerous studies investigating this condition have been performed using various model systems. To do so, investigators must be able to accurately measure myocardial performance in order to determine the degree of left ventricular function. In this model development study, we employ a wireless telemetry system purchased from Data Sciences International to continuously assess left ventricular function in the rabbit model.
We surgically implanted pressure-sensitive catheters fitted to wireless radio-transmitters into the left ventricle of Dutch-belted rabbits. Following recovery of the animals, we continuously recorded indices of cardiac contractility and ventricular relaxation at baseline for a given time period. The telemetry system allowed us to continuously record baseline left ventricular parameters for the entire recording period. During this time, the animals were unrestrained and fully conscious. The values we recorded are similar to those obtained using other reported methods.
The wireless telemetry system can continuously measure left ventricular pressure, cardiac contractility, and cardiac relaxation in the rabbit model. These results, which were obtained just as baseline levels, substantiate the need for further validation in this model system of left ventricular assessment.
In the case of left ventricular (LV) dysfunction, the myocardium surrounding the left ventricle is functionally disrupted, thereby causing impaired contraction and/or relaxation. The result is ineffective pumping and inadequate blood flow. Impaired left ventricular systolic function and/or impaired left ventricular diastolic function leads to congestive heart failure (CHF), which is a condition that is increasing in prevalence worldwide [1–3]. Consequently, LV dysfunction is a topic under intense investigation by the scientific community.
The degrees of contraction and relaxation are both used to assess overall LV function. With regards to the contractile phase, many have used cardiac contractility as a factor to gauge LV systolic function [4–7]. Myocardial contractility is the intrinsic ability of cardiac muscle to develop force at a given muscle length, and it increases upon sympathetic stimulation. Frequently, cardiac contractility is expressed as the maximum change in pressure divided by the change in time (dP/dtmax) [6, 8–11], and it denotes the maximum rate of increase in pressure during isovolumic contraction. Another parameter reported as a useful index of contractility is Vmax [12–14]. This is the maximal velocity of contractile shortening, and it has the added advantage of being load-independent. Ventricular relaxation, on the other hand, is used to assess LV diastolic function. It is often times expressed either as the maximum (negative) change in pressure divided by the change in time (-dP/dtmax) [15–19], which represents the decrease in pressure during isovolumic relaxation, or as Tau [16, 18, 20, 21], the time constant of LV relaxation. Since determining ventricular contractility and relaxation requires one to know ventricular pressure during full cardiac cycles, investigators, using various animal models, utilize pressure-sensitive catheters which are implanted into the animals' ventricle [22–26]. In an ideal model system, the catheterization is both well-tolerated by the animal as well as accurate in pressure reading. Moreover, an ideal system allows for the animal to remain in an unrestrained, conscious state during monitoring.
In this model development study, we test the utilization of wireless telemetry for determining various LV parameters, including left ventricular pressure (LVP), cardiac contractility, and the degree of ventricular relaxation, in the rabbit model. This wireless system is beneficial in that the animal is not restrained with a wire harness and is fully conscious which more accurately mimics normal cardiovascular conditions. The rabbit model has been used extensively in cardiovascular research, however, to our knowledge there are no reports describing the use of wireless telemetry to monitor LV function in specifically this animal model. Here we present baseline data that demonstrates the usefulness and feasibility of wireless, telemetric monitoring for determining the degree of LV function in conscious, unrestrained Dutch-belted rabbits. Consequently, this model system warrants further validation using physiological challenges and/or pharmacological interventions.
Rabbits and housing
Specific-pathogen-free female Dutch-belted dwarf rabbits, 7 to 8 weeks in age and weighing 1.5 to 2.0 kg, were purchased from Myrtle's Rabbitry (Thompson Station, TN). The vendor's comprehensive health assessment, which includes serologic testing, indicated that the animals were free from Pasteurella multocida, Pasteurella pneumotropica, Bordetella bronchiseptica, Treponema cuniculi, Clostridium piliformis, cilia-associated respiratory bacillus, oral papillomavirus, arthropod ectoparasites, helminth endoparasites, and protozoans. Upon delivery, the animals were pair- or singly housed at the University of Texas Medical Branch Animal Resources Center in stainless steel, ventilated rabbit racks (Allentown, Allentown, NJ) and allowed to acclimate for one week. The animal room was maintained on a 12:12-hr light:dark cycle, with the temperature range at 19 to 22°C and the humidity between 30% and 70%. The rabbits were fed approximately 170 g commercial chow (Rabbit Diet 5321, LabDiet, Richmond, IN) daily and given water ad libitum. All animal procedures were conducted under protocols approved by the University of Texas Medical Branch Institutional Animal Care and Use Committee.
Telemetry system and surgical implantation
The telemetry equipment, purchased from Data Sciences International (DSI) (St. Paul, MN), included an implantable transmitter (model TL11M3-D70-PCTP), a receiver (model RMC1), a data processing device (Data Exchange Matrix), and an ambient pressure reference monitor (APR-1). The transmitter has two pressure catheters and two biopotential leads, however, only one pressure catheter was used. The unused catheter/leads were immobilized near the body of the transmitter. The pressure catheter is a 14-cm fluid filled catheter with a terminal sensing region containing a non-compressible fluid and a plug of biocompatible gel. This sensing region relays pressure waves to the DSI transmitter. Calibration of the transmitter is accomplished by placing it into a sealed pressure chamber with measurements taken at 750, 850, and 950 mmHg. The output is read as a raw frequency output and is used as the calibration values for the transmitter. The data for each rabbit were recorded approximately two weeks after surgery, with the exact numbers of days being dependent on the animals' level of recovery. The data acquisition system, which has a sampling frequency of 500 Hz, was programmed to record data continuously for 72 h, with the data recorded every 20 s. This was later used to compute 1-h moving averages. The parameters that were continuously monitored were heart rate, mean left ventricular pressure (LVP), left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), dP/dtmax, Vmax, -dP/dtmax, and Tau. LVSP is defined as the peak pressure after the detected dP/dtmax, while LVEDP is defined as the pressure at the end of diastole. Also, Tau is defined as the relaxation time constant from -dP/dtmax to the point where the pressure has dropped 66% of the distance from systolic to diastolic pressure. All data was computed using an analysis program (Dataquest 4.1, Data Sciences International, St. Paul, MN), and all graphs were prepared in Excel (Microsoft).
The rabbits were given ketamine-HCl (50 mg/kg IM) (Fort Dodge Animal Health, Fort Dodge, IA), buprenorphine (0.05 mg/kg IM) (Hospira, Inc, Lake Forest, IL), and glycopyrrolate (0.1 mg/kg IM) (American Regent, Inc., Shirley, NY) as pre-anesthetics prior to intubation. They were intubated with a 2.5 mm cuffed endotracheal tube using the blind technique, and they were inducted under 3% isoflurane (Webster Veterinary, Sterling, MA) and maintained at 2-2.5% during the surgical procedure. A catheter was placed in the left ear vein for fluid replacement with Lactated Ringers solution (Baxter, Deerfield, IL) at a rate of 30 ml/kg/hr. The temperature, ECG, blood pressure and pulse oximeter probes were placed on the animals and measured using the Surgivet (Smith Medical, Inc., Waukesha, WI). The rabbits were supplemented with heat using a heating pad (Gaymar Industries, Inc., Orchard Park, NY). Once the thoracic cavity was opened, the rabbit was ventilated at 15 to 30 breaths per minute until the cavity was closed.
The rabbits, which were singly housed following surgical implantation, were checked for any abnormal breathing, pain, or distress. Following the surgery, heat was provided until the rabbits regained normal body temperature. Buprenorphine was given for the first 48 hours, and the animals were monitored very closely until they were eating and drinking normally (approximately 2 weeks).
Heart rate and Left ventricular pressures
Indices of contractility and relaxation
Contractility and relaxation indices of rabbits on each day for 3 days
3081 ± 42
2908 ± 50
2735 ± 27
3702 ± 33
3879 ± 51
4126 ± 74
2116 ± 23
2342 ± 42
2228 ± 34
1338 ± 19
1138 ± 10
1098 ± 9
1615 ± 43
1534 ± 38
1535 ± 36
1339 ± 20
1489 ± 29
1309 ± 30
2385 ± 32
2122 ± 34
2099 ± 17
2785 ± 23
2978 ± 30
3067 ± 52
2464 ± 35
2811 ± 45
2742 ± 48
29 ± 0.4
31 ± 0.2
31 ± 0.2
24 ± 0.3
23 ± 0.2
22 ± 0.1
54 ± 0.4
49 ± 0.9
50 ± 0.4
Telemetry has been used extensively in various animal models in the past with the purpose of investigating cardiovascular disorders. The use of telemetry in the murine model was recently reported by investigators assessing right ventricular systolic pressure and heart rate in mice treated with an experimental therapeutic aimed at reducing pulmonary hypertension . In this murine study, the investigators introduced a pressure catheter into the right ventricle of the mice by way of the right jugular vein. Likewise, telemetry has been used for determining cardiac function, specifically contractility, in rats . In this case, the investigators were able to determine the rats' QA interval, an indirect indicator of contractility, by surgically implanting ECG and pressure leads. In addition to the rodent models, animal models involving higher organisms have also been used in conjunction with telemetry, namely dogs, pigs, and non-human primates [23, 25, 26]. Nonetheless, we report here using dwarf Dutch-belted rabbits which have the added advantages of being more similar to humans relative to rodents, and being more cost-effective and easy to handle relative to non-human primates and other higher organisms. There are past studies which involved surgically placing a pressure transducer in the heart of rabbits in order to evaluate LV function, however, in these cases, the systems allowed only limited movement since the transducers used were physically connected to an acquisition/storage system [24, 29]. Our model system allows the rabbits to remain unrestrained and fully conscious.
The values we recorded for the various parameters are similar to those reported in previous studies that used either an alternate method, such as echocardiography, or different non-wireless hardware, for assessing LV function in naïve rabbits [24, 29–31]. Any differences between our values and the values of these previous studies could be attributed to the use of a different rabbit strain, which often times was the New Zealand White strain. Some discrepancies could also be due to the fact that in some of these previous studies the animals were under anesthesia while being monitored. Additionally, the surgical procedure we present here, while beneficial for directly measuring LV pressures, could bring about slightly altered values for LV function, which is probable with an invasive procedure of this type. Also, proper placement of the pressure catheter tip, which is directly at the apex, is crucial for assuring that the tip is directly in the ventricular chamber. Any contact of the catheter tip with the muscle wall (can occur due to animal movement), or any blood-flow obstruction, could lead to abnormal pressure waveforms which in turn would result in atypical pressure values. This could have occurred with rabbit 3, which would possibly explain the lower dP/dtmax relative to the remaining two animals and the slightly different waveform. Then again, both the -dP/dtmax and Vmax from rabbit 3 was closely similar to rabbits 1&2, suggesting that the pressure waveform of rabbit 3 used to calculate the parameters was normal. Also worth noting is that our dP/dtmax (and -dP/dtmax) are derived from pressure measurements which makes it load-dependent. Fortunately, this system is able to denote contractility as Vmax as well, which is reported to be a load-independent variable . Future studies that involve altering cardiac physiology (heart rate, preload, afterload, and etc.) by means of drug administration and/or surgical manipulation would be beneficial for testing this model system further.
The DSI telemetry system allowed us to record various parameters (at baseline) that are indicative of the degree of LV function. The values we present here were recorded in Dutch-belted rabbits that were both fully conscious and unrestrained. Monitoring the animals in this state lessens the role that either stress due to restraint or anesthesia might play in attaining accurate results. Overall, this report supports the use of wireless telemetry in assessing LV function in the rabbit model.
List of abbreviations
Congestive heart failure
Data Sciences International
Left ventricular pressure
Left ventricular systolic pressure
Left ventricular end diastolic pressure.
This work was supported by the U.S. Army (DAMD170210699) and the National Institute of Health (NO1-AI-30065).
- Dimengo JM: Surgical alternatives in the treatment of heart failure. AACN Clin Issues. 1998, 9 (2): 192-207. 10.1097/00044067-199805000-00004.PubMedView ArticleGoogle Scholar
- Gould PA, Kaye DM: Clinical treatment regimens for chronic heart failure: a review. Expert Opin Pharmacother. 2002, 3 (11): 1569-1576. 10.1517/146565188.8.131.529.PubMedView ArticleGoogle Scholar
- Ikram H: Identifying the patient with heart failure. J Int Med Res. 1995, 23 (3): 139-153.PubMedGoogle Scholar
- Brothers RM, Bhella PS, Shibata S, Wingo JE, Levine BD, Crandall CG: Cardiac systolic and diastolic function during whole body heat stress. Am J Physiol Heart Circ Physiol. 2009, 296 (4): H1150-1156. 10.1152/ajpheart.01069.2008.PubMedPubMed CentralView ArticleGoogle Scholar
- Mullner M, Domanovits H, Sterz F, Herkner H, Gamper G, Kurkciyan I, Laggner AN: Measurement of myocardial contractility following successful resuscitation: quantitated left ventricular systolic function utilising non-invasive wall stress analysis. Resuscitation. 1998, 39 (1-2): 51-59. 10.1016/S0300-9572(98)00122-1.PubMedView ArticleGoogle Scholar
- Royse CF, Connelly KA, MacLaren G, Royse AG: Evaluation of echocardiography indices of systolic function: a comparative study using pressure-volume loops in patients undergoing coronary artery bypass surgery. Anaesthesia. 2007, 62 (2): 109-116. 10.1111/j.1365-2044.2006.04911.x.PubMedView ArticleGoogle Scholar
- Tomson CR: Echocardiographic assessment of systolic function in dialysis patients. Nephrol Dial Transplant. 1990, 5 (5): 325-331.PubMedView ArticleGoogle Scholar
- Dhainaut JF, Bricard C, Monsallier FJ, Salmon O, Bons J, Fourestie V, Schlemmer B, Carli A: Left ventricular contractility using isovolumic phase indices during PEEP in ARDS patients. Crit Care Med. 1982, 10 (10): 631-635. 10.1097/00003246-198210000-00002.PubMedView ArticleGoogle Scholar
- Dowell RT, Houdi AA: Aortic peak flow velocity as an index of myocardial contractility in the conscious rat. Methods Find Exp Clin Pharmacol. 1997, 19 (8): 533-539.PubMedGoogle Scholar
- Mason DT, Braunwald E, Covell JW, Sonnenblick EH, Ross J: Assessment of cardiac contractility. The relation between the rate of pressure rise and ventricular pressure during isovolumic systole. Circulation. 1971, 44 (1): 47-58.PubMedView ArticleGoogle Scholar
- Schmidt HD, Hoppe H: Influence of the contractile state of the heart of the preload dependence of the maximal rate of intraventricular pressure rise dP/dt max. Cardiology. 1978, 63 (2): 112-125. 10.1159/000169888.PubMedView ArticleGoogle Scholar
- Falsetti HL, Andersen MN: Aneurysm of the membranous ventricular septum producing right ventricular outflow tract obstruction and left ventricular failure. Chest. 1971, 59 (5): 578-580. 10.1378/chest.59.5.578.PubMedView ArticleGoogle Scholar
- Pollack GH: Maximum velocity as an index of contractility in cardiac muscle. A critical evaluation. Circ Res. 1970, 26 (1): 111-127.PubMedView ArticleGoogle Scholar
- Sonnenblick EH, Brutsaert DL: V max: its relation to contractility of heart muscle. Cardiology. 1972, 57 (1): 11-15. 10.1159/000169499.PubMedView ArticleGoogle Scholar
- Ahmed SS, Regan TJ: Assessment of left ventricular contractile performance from isovolumic relaxation phase in man. Cardiology. 1981, 68 (1): 1-18.PubMedView ArticleGoogle Scholar
- Hirota Y: A clinical study of left ventricular relaxation. Circulation. 1980, 62 (4): 756-763.PubMedView ArticleGoogle Scholar
- Ohte N, Narita H, Hashimoto T, Kobayashi K, Akita S, Fujinami T: Left ventricular isovolumic relaxation flow and left ventricular systolic performance. J Am Soc Echocardiogr. 1995, 8 (5 Pt 1): 690-695.PubMedView ArticleGoogle Scholar
- Voon WC, Su HM, Yen HW, Lin TH, Lai WT, Sheu SH: Validation of isovolumic relaxation flow propagation velocity as an index of ventricular relaxation. Ultrasound Med Biol. 2007, 33 (7): 1098-1103. 10.1016/j.ultrasmedbio.2007.01.010.PubMedView ArticleGoogle Scholar
- Yellin EL, Nikolic S, Frater RW: Left ventricular filling dynamics and diastolic function. Prog Cardiovasc Dis. 1990, 32 (4): 247-271. 10.1016/0033-0620(90)90016-U.PubMedView ArticleGoogle Scholar
- Scalia GM, Greenberg NL, McCarthy PM, Thomas JD, Vandervoort PM: Noninvasive assessment of the ventricular relaxation time constant (tau) in humans by Doppler echocardiography. Circulation. 1997, 95 (1): 151-155.PubMedView ArticleGoogle Scholar
- Varma SK, Owen RM, Smucker ML, Feldman MD: Is tau a preload-independent measure of isovolumetric relaxation?. Circulation. 1989, 80 (6): 1757-1765. 10.1161/01.CIR.80.6.1757.PubMedView ArticleGoogle Scholar
- Adeyemi O, Roberts S, Harris J, West H, Shome S, Dewhurst M: QA interval as an indirect measure of cardiac contractility in the conscious telemeterised rat: model optimisation and evaluation. J Pharmacol Toxicol Methods. 2009, 60 (2): 159-166. 10.1016/j.vascn.2009.03.006.PubMedView ArticleGoogle Scholar
- Norton K, Iacono G, Vezina M: Assessment of the pharmacological effects of inotropic drugs on left ventricular pressure and contractility: an evaluation of the QA interval as an indirect indicator of cardiac inotropism. J Pharmacol Toxicol Methods. 2009, 60 (2): 193-197. 10.1016/j.vascn.2009.05.008.PubMedView ArticleGoogle Scholar
- Preckel B, Schlack W, Heibel T, Rutten H: Xenon produces minimal haemodynamic effects in rabbits with chronically compromised left ventricular function. Br J Anaesth. 2002, 88 (2): 264-269. 10.1093/bja/88.2.264.PubMedView ArticleGoogle Scholar
- Stubhan M, Markert M, Mayer K, Trautmann T, Klumpp A, Henke J, Guth B: Evaluation of cardiovascular and ECG parameters in the normal, freely moving Gottingen Minipig. J Pharmacol Toxicol Methods. 2008, 57 (3): 202-211. 10.1016/j.vascn.2008.02.001.PubMedView ArticleGoogle Scholar
- Yao JA, Feldman HS, Illenberger A, Littell T, Schnee L, Yates D: Evaluation of electrocardiograms recorded in cynomolgus monkeys with short- and long-term intracardiac lead implantations. J Pharmacol Toxicol Methods. 2009, 60 (2): 185-192. 10.1016/j.vascn.2009.05.009.PubMedView ArticleGoogle Scholar
- Suckow M, Douglas F: Important Biological Features. The Laboratory Rabbit. Edited by: Suckow MA. 1997, Notre Dame: CRC Press, 1-10.Google Scholar
- Weissmann N, Hackemack S, Dahal BK, Pullamsetti SS, Savai R, Mittal M, Fuchs B, Medebach T, Dumitrascu R, Eickels MV: The soluble guanylate cyclase activator HMR1766 reverses hypoxia-induced experimental pulmonary hypertension in mice. Am J Physiol Lung Cell Mol Physiol. 2009Google Scholar
- Signolet IL, Bousquet PP, Monassier LJ: Improvement of cardiac diastolic function by long-term centrally mediated sympathetic inhibition in one-kidney, one-clip hypertensive rabbits. Am J Hypertens. 2008, 21 (1): 54-60. 10.1038/ajh.2007.9.PubMedView ArticleGoogle Scholar
- Bauersachs J, Hiss K, Fraccarollo D, Laufs U, Ruetten H: Simvastatin improves left ventricular function after myocardial infarction in hypercholesterolemic rabbits by anti-inflammatory effects. Cardiovasc Res. 2006, 72 (3): 438-446. 10.1016/j.cardiores.2006.08.014.PubMedView ArticleGoogle Scholar
- Mahaffey KW, Raya TE, Pennock GD, Morkin E, Goldman S: Left ventricular performance and remodeling in rabbits after myocardial infarction. Effects of a thyroid hormone analogue. Circulation. 1995, 91 (3): 794-801.PubMedView ArticleGoogle Scholar
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