Spliceosomal intron size expansion in domesticated grapevine (Vitis vinifera)
© Jiang et al; licensee BioMed Central Ltd. 2011
Received: 13 January 2011
Accepted: 8 March 2011
Published: 8 March 2011
Spliceosomal introns are important components of eukaryotic genes as their structure, sizes and contents reflect the architecture of gene and genomes. Intron size, determined by both neutral evolution, repetitive elements activities and potential functional constraints, varies significantly in eukaryotes, suggesting unique dynamics and evolution in different lineages of eukaryotic organisms. However, the evolution of intron size, is rarely studied. To investigate intron size dynamics in flowering plants, in particular domesticated grapevines, a survey of intron size and content in wine grape (Vitis vinifera Pinot Noir) genes was conducted by assembling and mapping the transcriptome of V. vinifera genes from ESTs to characterize and analyze spliceosomal introns.
Uncommonly large size of spliceosomal intron was observed in V. vinifera genome, otherwise inconsistent with overall genome size dynamics when comparing Arabidopsis, Populus and Vitis. In domesticated grapevine, intron size is generally not related to gene function. The composition of enlarged introns in grapevines indicated extensive transposable element (TE) activity within intronic regions. TEs comprise about 80% of the expanded intron space and in particular, recent LTR retrotransposon insertions are enriched in these intronic regions, suggesting an intron size expansion in the lineage leading to domesticated grapevine, instead of size contractions in Arabidopsis and Populus. Comparative analysis of selected intronic regions in V. vinifera cultivars and wild grapevine species revealed that accelerated TE activity was associated with grapevine domestication, and in some cases with the development of specific cultivars.
In this study, we showed intron size expansion driven by TE activities in domesticated grapevines, likely a result of long-term vegetative propagation and intensive human care, which simultaneously promote TE proliferation and repress TE removal mechanisms such as recombination. The intron size expansion observed in domesticated grapevines provided an example of rapid plant genome evolution in response to artificial selection and propagation, and may shed light on the important genomic changes during domestication. In addition, the transcriptome approach used to gather intron size data significantly improved annotations of the V. vinifera genome.
Eukaryotic genes contain spliceosomal introns that are post-transcriptionally removed by the spliceosome, an RNA-protein complex . The length, position and phase of spliceosomal introns are important components to the evolution of genome architecture . However, most intronic regions are often considered 'junk' DNA similar to intergenic regions or other non-coding sequences. Increasing scrutiny of genomic data has revealed many conserved non-coding sequences (CNS) in the non-coding DNA of both plant and animal genomes . CNS may conduct important functions and experience selective constraints . One major function of CNS is regulating gene expression via interactions between small DNA motifs and transcription machinery (transcription factors and RNA polymerase) . The regulatory motifs are enriched in 5' promoter regions and the first introns of plant and animal genes .
Plant genomes usually have compact genes due to small introns . Arabidopsis, the plant model system first completely sequenced, has an average gene size of 2000 bp and average intron size of 180 bp . Intron size is hypothesized to be constrained by energy use in transcription, as large introns may require more energy to be transcribed and spliced, so there is selection against introns of excessive size . However, in certain genes, selection against intron size may be counteracted by a selective preference for bigger introns with more regulatory elements and finer control of gene expression .
Transposable elements (TEs) are known to be major components of plant genomes . The origin and proliferation of TEs in plant genomes shaped plant genome dynamics and genetic diversity. Unlike the human genome in which TEs are predominantly in introns , plant TEs are usually found in intergenic regions, possibly a result of strong purifying selection against TE insertions in exons and introns . The observed plant genome size expansions of cereals are thought to be results of whole-genome duplications and TE invasions in non-coding regions . However, in a few cases, TE insertions in introns altered temporal and spatial expression patterns of specific genes. The insertions caused significant genetic and phenotypic changes and seemed to be preserved by natural selection . During plant domestication, key traits selected by human may be the result of genetic diversity generated by TE insertions .
To further investigate intron size evolution in plant genomes, we present a novel approach to identify and analyze introns of large size using genomic data for a domesticated grapevine cultivar (Vitis vinifera Pinot Noir). The specific objectives are to investigate the size distribution of introns; relationship between intron size and gene function, determine the contents of introns and examine the associations between intron size evolution and grapevine domestication. Generally, we show that introns of truly extraordinary size are widespread in cultivated grapevines, a phenomenon not observed in other plant genomes.
EST collection, processing and assembly
In total, 353,748 publicly available EST sequences of V. vinifera were clustered into 26,111 clusters, 8,560 of which represented by only one EST sequence. The largest cluster is composed of 1,998 EST sequences. The distribution of cluster size suggested that one-third of V. vinifera genes are not expressed at very high levels (32.8% singletons) and a few (3 clusters contain more than 1000 ESTs) genes are highly expressed across the representative EST libraries. Among the 26,111 clusters, 5,678 clusters contain no intron, while 20,433 clusters were predicted to be spliced. Among all clusters, 17,059 clusters were predicted to encode proteins, in which 15,369 predicted proteins show homology to Arabidopsis proteins and 15,683 proteins show homology to Populus proteins (BLASTP, E-value < 0.001). BLAST searches against an EST database of other Vitis species showed that 15,089 clusters (60.5%) had high similarity hits in at least one other species of grapevine.
Identification and annotation of large genes and introns
The average intron size, predicted by the annotation of the first draft sequences of Vitis vinifera Pinot Noir, is slightly larger than other sequenced plants . After mapping the assembled consensus sequences to V. vinifera genomic regions, 2,697 introns having size between 3 kb and 100 kb were identified in 2,563 genes (10% of all assembled clusters, 13% of spliced clusters). Among these genes, 1,179 (46.0%) only have one EST sequence (singletons), a higher proportion than among all predicted genes from EST clusters (32.8%). This indicates that if the number of EST sequences in cluster is a good indictor of expression level, genes with large introns had lower expression level compared to all genes (46.0% vs 32.8% singletons).
Contents of large introns and TEs
In the 39 genes with identical exon/intron structure/number in Arabidopsis and Populus, repetitive elements comprised roughly 90% of the 74 intronic regions, in which 10 complete LTR retrotransposons were identified. Among the 74 introns, 1 intron contains only a LINE; 1 intron contains only LTR elements; 15 introns contain LINEs and other repetitive elements; 15 introns contain LTR elements and other repetitive elements; 5 introns contain LTR, LINEs and other repetitive elements; 34 introns contain only other repetitive elements; and 3 introns do not contain repetitive elements. According to a BLAST search against the V. vinifera genome, 'other repetitive elements' were highly repetitive within V. vinifera and likely represented un-classified or unnamed grapevine-specific TEs. The three introns without any identified repetitive elements all contain LINE-like ORFs otherwise highly repetitive in the V. vinifera genome.
Characterization of selected introns in wild grapevine species and V. vinifera cultivars
Transcriptome and intron identification
Genome-wide intron analysis has only been done in very few plants . In this study, we identified introns on a whole genome scale by combining a large EST data set and genomic sequences in an automated workflow. This approach has been rarely used before because sufficient EST and genomic data are needed but available for only a few plant species . Domesticated grapevine ranks in the top 25 taxa with the largest collections of ESTs and is the fourth whole plant genome sequence available, making it ideal for the EST mapping approach described here. EST cluster number in this study is higher than the predicted gene number of V. vinifera , suggesting that gene prediction based on EST consensus sequences covered a gene range comparable to genomic annotation. By manually checking selected matches between EST consensus sequences and genomic regions, the rate of correct mapping is 100%. Therefore, our workflow accurately identified the corresponding genomic regions of EST consensus sequences, thus correct intron positions and sizes.
Annotation errors such as splitting single gene into multiple ones as a result of unsually long introns, were revealed by comparing ESTs to current annotations (Figure 1). This was expected in two ways: correct prediction of genes in genomic sequences is very sensitive to gene size because large size genes are more likely to be predicted incorrectly due to fragmentation ; the large introns present in these genes tend to cause prediction algorithms to split exons at both ends of large introns into two separate genes. Incorporating ESTs into gene prediction algorithms can clearly refine genome annotation as suggested by Coyne et al. .
Properties of genes with large introns
Large size introns are expected to require more energy to be transcribed and spliced, so large introns may decrease gene expression (energy cost hypothesis) . Such hypothesis was supported by this study if we consider that the number of ESTs is a good indicator of gene expression level. However, there is evidence against this hypothesis in genes expressed in specific mammal organs/tissues  and plant genomes .
In contrast, gene size and/or intron size seems to be not related to gene function in this study. Among identified large genes, there is no particularly overrepresented gene family. There are still about 20% of expanded intronic regions not accounted for by similarity searches or repeat masking, suggesting unique sequences in introns are also evolving. Most of these unique sequences may be evolving neutrally since there may not be any selective constraint on them. However, a few of them may contain very important regulatory elements controlling gene expression patterns thus under selective constraints.
Intron size expansion, TEs, grapevine evolution and domestication
Comparisons of genome sizes and intron sizes between Populus and Vitis revealed that intron sizes has increased much more than genome size. Either grapevine specific intron expansion or Populus specific intron contraction can explain the intron size difference between the two plants. The young LTR retrotransposons and unique presence of these TEs in domesticated grapevine both suggested that an intron size expansion is more likely to be the case. Wendel et al. [18, 24] proposed that intron size dynamics may be decoupled with genome size evolution by showing that intron size stayed static in multiple rounds of genome expansion and contraction in cotton (Gossypium). In contrast, we showed that Vitis intron size has experienced a 5-fold expansion compared to Populus, with either unknown fluctuation in genome size, or none at all. Intron size dynamics in Vitis seem to be decoupled from genome size evolution but our data suggested expansion instead of conservation of intron size.
A major force behind the intron size expansion in grapevine is the proliferation of TEs, consistent with previous observations . Among various TEs, LTR retrotransposons provides a proxy to estimate temporal aspects of their activities due to their unique structure. Several studies have estimated the age of LTR retrotransposon insertions in plant genomes such as rice, Medicago and maize [25–27]. Peaks of LTR retrotransposon activity were identified including some that were very recent and possibly related to plant domestication [28, 29]. Vitte and Panaud  suggested that plant LTR retrotransposons evolved following a 'burst and contraction' model, which could explain the observed 'peaks' of LTR retrotransposon activities in various plant genomes. Different age estimates of Vitaceae family all placed its origin at around 110 million years ago [31–33]. As a result, LTR retrotransposon bursts observed in the introns are much younger than the divergence of Vitales (with a single family Vitaceae) from the common ancestor with rosids, suggesting that increased retrotransposon activity, as well as intron size expansion, is also associated with unique evolutionary changes along the grapevine lineage such as recent domestication.
V. vinifera expanded introns contain significantly more identical LTR pairs, suggesting that more young LTR retrotransposons have been preserved in introns of domesticated grapevine (Figure 4a). Here we suggest that the most recent burst of LTR retrotransposons in grapevine may be associated with domestication, either as a result of artificial selection on the nearby genes or genomic regions (local effect), or a non-adaptive by-product of the domestication bottleneck and specific reproduction modes in domesticated grapevines (genome-wide effect). There is no enrichment of any particular gene families in genes with TE invaded introns. Threrfore it is very unlikely that all of the TEs and introns, or nearby genes with diverse functions, which are randomly distributed in genome, were selected by humans in the domestication process. Instead, LTR retrotransposons in the most recent burst were more likely to be preserved in introns than previous bursts due to vegetative clonal propagation of domesticated grapevine plants. Recent and variety-specific TE insertions in introns were discovered in our characterization of large introns from several commonly cultivated grapevine varieties, suggesting that initial TE invasion of introns spread with clonal propagations (see results, Table 1) . This may be explained by the following reasons: first, somaclonal propagation of plants (or tissue culture) was suggested to promote or induce LTR retrotransposon activity [35, 36]. Domesticated plants with vegetative propagation may generate more LTR retrotransposons than wild species and non-clonal cultivated plants. Second, plant genome expansions caused by TE proliferation are counteracted by rapid DNA removal as a result of recombination . However, due to a domestication bottleneck and vegetative propagation, the linkage disequilibrium (LD) in cultivated grapevines can be found at extremely long distance compared to wild species , suggesting that recombination is repressed in large chunks of chromosomes. TEs, in particular LTR retrotransposons, in domesticated grapevines, may not be removed as effectively as in wild species. Third, LTR retrotransposons, as mutagenesis factors, may cause deleterious mutations if inserted in genes. The mutants were selected against and removed from populations very quickly in natural environments . In domesticated plants, intensive human care produces an environment with relaxed selective constraints, in which LTR retrotransposons invaded genes were more likely to persist. Particularly, vegetatively propagated cultivars such as domesticated grapevine have a uniform genetic background as a result of clonal reproduction to preserve desired traits. This reproduction mode facilitated the spread and fixation of introns with LTR retrotransposons (and other TEs) to the entire clonal population or variety. This may be the major reason why excessive TEs were not observed within genes of other plant genomes, including domesticated plants without clonal propagation such as rice [40, 41].
In this study, the intron size dynamics in domesticated grapevine suggested that intron size expansion, mainly caused by TE invasions, was associated with the plant's unique and recent evolutionary history. Intron size expansion and burst of TE activity may all be related to major evolutionary changes associated with domestication in grapevine. Investigation of intron size expansion not only reveals a unique pattern of plant genome dynamics, but also raises an interesting question: what is the role of introns, a somewhat neglected portion of the genome, in plant genome architecture, function and evolution?
EST data collection, processing and assembly
All currently available V. vinifera Expressed Sequence Tags (ESTs) were obtained from NCBI GenBank using the PartiGene package . Only V. vinifera ESTs were included in this study, including over 140 varieties and cultivars. Vector and poly-A sequences were trimmed by the built-in functions of PartiGene. Cleaned EST sequences were clustered using both CLOBB (the default clustering method of PartiGene ) and TGICL . Both methods employ megaBLAST  searches to produce clusters based on sequence similarity. For both methods, a similarity cut-off of 99% and greater than 100 bp overlap were employed. Both approaches yielded similar numbers of EST clusters and the clustering results of TGICL were adopted for further analyses. The clusters were assembled and the consensus sequences for each cluster were predicted by PHRAP . The protein coding potential of the clusters were predicted by ESTScan .
Identification of large genes and introns
EST consensus sequences were mapped to genomic sequences of V. vinifera Pinot Noir  to identify genomic locations and exon/intron structures of the predicted genes. The mapping process was performed with BLAT, by which ESTs or cDNAs were aligned to genomic regions with near identity . Most alignment gaps in BLAT results represent intronic regions. Genes with putative large introns were selected by two criteria: first, the similarity score from BLAT search is higher than 99%; second, the mapped genomic region (excluding introns) cover 95% of the EST consensus sequence. These criteria ensure that predicted gene sequences were mapped to the correct genomic positions. The process was automated with a Perl script for parsing BLAT results, in which the gap sizes were calculated and gap sequences (size range 3 kb~100 kb) and coding regions associated with them were extracted. Extracted gap regions were manually inspected for splicing signals. Gaps with canonical donor and acceptor splice sites (5'-end GT and 3'-end AG) were considered introns. The entire process was also conducted on Arabidopsis and Populus ESTs and genomes to characterize large introns in those plant species.
Genes with putative large introns were submitted to the following analysis: 1, the coding regions were predicted and translated into protein sequences by ESTScan ; 2, predicted coding regions were compared to Genoscope annotations by similarity searches and manual corrections; 3, BLAST searches were conducted using the predicted coding regions as queries against an EST database of Vitis species other than V. vinifera. Presence of high similarity hits in non-V. vinifera EST database suggested that the genes have EST data in at least two closely related species, thus they are very likely truly expressed; 4. BLAST searches using predicted protein sequences were conducted against the Arabidopsis and Populus genomic databases to identify the homologous genes in the two plant genomes as an indicator of possible function. In addition, the predicted V. vinifera proteins were subjected to HMM search of protein functional domains against Pfam database .
Analysis of large intron contents and selected individual introns
Identified large introns (3 kb~100 kb-long) were subjected to the following analysis: 1, the intronic sequences were screened by RepeatMasker  to identify repetitive elements using an Arabidopsis repetitive element library; 2, the introns without any repetitive elements detectable by RepeatMasker were subjected to BLAST searches against the V. vinifera genome. Presence of large number of high scoring hits (cut-off E-value = 0.001) suggests grapevine-specific repetitive elements. 3. Full length LTR retrotransposons in intronic regions were identified by LTR_FINDER . The sequence similarity between two LTR regions were used to estimate the age of LTR retrotransposon insertions using the methods described by SanMiguel et al. , with the JC substitution model used to correct for multiple substitutions in LTR regions . All statistical analyses were conducted with the statistical package R .
To characterize homologous introns in other Vitis species and varieties, intronic regions that met the following criteria were chosen: 1, there is only one repetitive element in the intron, suggesting a relatively recent expansion; 2, the total intron size without repetitive elements does not exceed 3 kb (to facilitate PCR amplifications). Primers were designed for the flanking exon sequence as well as conserved regions within the repetitive elements to allow scoring either the presence or absence of the elements in a given intron (primer sequences available upon request). PCR amplifications of selected intronic regions were performed for one individual each of six wild grapevine species: V. rotundifolia, V. californica, V. girdiana, V. aestivalis and V. labrusca, V. jacquemontii, and seven V. vinifera cultivars: Cabernert Sauvignon, Chardonnay, Dolcetto, Pinot Noir, Riesling, Sangiovese and Zinfandel. Alleles with or without TE insertions were determined by the size of the PCR product.
This work was supported by Alabama Experimental Program to Stimulate Competitive Research [RII (05-08): NSF EPS-04476752] to K.J.
- Chow L, Gelinas R, Broker T, Roberts R: An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA. Cell. 1977, 12 (1): 1-8. 10.1016/0092-8674(77)90180-5.PubMedView ArticleGoogle Scholar
- Irimia M, Roy S: Spliceosomal introns as tools for genomic and evolutionary analysis. Nucleic Acids Res. 2008, 36 (5): 1703-12. 10.1093/nar/gkn012.PubMedPubMed CentralView ArticleGoogle Scholar
- Elgar G: Pan-vertebrate conserved non-coding sequences associated with developmental regulation. Brief Funct Genomic Proteomic. 2009, 8 (4): 256-65. 10.1093/bfgp/elp033.PubMedView ArticleGoogle Scholar
- Keightley P, Gaffney D: Functional constraints and frequency of deleterious mutations in noncoding DNA of rodents. Proc Natl Acad Sci USA. 2003, 100: 13402-13406. 10.1073/pnas.2233252100.PubMedPubMed CentralView ArticleGoogle Scholar
- Woolfe A, Goodson M, Goode DK, Snell P, McEwen GK, Vavouri T, Smith SF, North P, Callaway H, Kelly K, Walter K, Abnizova I, Gilks W, Edwards YJ, Cooke JE, Elgar G: Highly conserved non-coding sequences are associated with vertebrate development. PLoS Biol. 2005, 3 (1): e7-10.1371/journal.pbio.0030007.PubMedPubMed CentralView ArticleGoogle Scholar
- Majewski J, Ott J: Distribution and characterization of regulatory elements in the human genome. Genome Res. 2002, 12: 1827-1836. 10.1101/gr.606402.PubMedPubMed CentralView ArticleGoogle Scholar
- Hong X, Scofield D, Lynch M: Intron size, abundance, and distribution within untranslated regions of genes. Mol Biol Evol. 2006, 23 (12): 2392-404. 10.1093/molbev/msl111.PubMedView ArticleGoogle Scholar
- Arabidopsis Genome Initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature. 2000, 408 (6814): 796-815. 10.1038/35048692.View ArticleGoogle Scholar
- Castillo-Davis C, Mekhedov S, Hartl D, Koonin E, Kondrashov F: Selection for short introns in highly expressed genes. Nat Genet. 2002, 31 (4): 415-8.PubMedGoogle Scholar
- Marais G, Nouvellet P, Keightley P, Charlesworth B: Intron size and exon evolution in Drosophila. Genetics. 2005, 170 (1): 481-5. 10.1534/genetics.104.037333.PubMedPubMed CentralView ArticleGoogle Scholar
- Kumar A, Bennetzen J: Plant retrotransposons. Annu Rev Genet. 1999, 33: 479-532. 10.1146/annurev.genet.33.1.479.PubMedView ArticleGoogle Scholar
- Wong G, Passey D, Yu J: Most of the human genome is transcribed. Genome Res. 2001, 11 (12): 1975-7. 10.1101/gr.202401.PubMedView ArticleGoogle Scholar
- Bennetzen J: Transposable element contributions to plant gene and genome evolution. Plant Molecular Biology. 2000, 42 (1): 251-69. 10.1023/A:1006344508454.PubMedView ArticleGoogle Scholar
- Messing J, Bennetzen J: Grass genome structure and evolution. Genome Dyn. 2008, 4: 41-56. full_text.PubMedView ArticleGoogle Scholar
- Lempe J, Balasubramanian S, Sureshkumar S, Singh A, Schmid M, Weigel D: Diversity of flowering responses in wild Arabidopsis thaliana strains. PLoS Genet. 2005, 1 (1): 109-18. 10.1371/journal.pgen.0010006.PubMedView ArticleGoogle Scholar
- This P, Lacombe T, Cadle-Davidson M, Owens C: Wine grape (Vitis vinifera L.) color associates with allelic variation in the domestication gene VvmybA1. Theor Appl Genet. 2007, 114 (4): 723-30. 10.1007/s00122-006-0472-2.PubMedView ArticleGoogle Scholar
- Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyère C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, Le Clainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M, Pezzotti M, Lecharny A, Scarpelli C, Artiguenave F, Pè ME, Valle G, Morgante M, Caboche M, Adam-Blondon AF, Weissenbach J, Quétier F, Wincker P, French-Italian Public Consortium for Grapevine Genome Characterization: The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature. 2007, 449 (7161): 463-7. 10.1038/nature06148.PubMedView ArticleGoogle Scholar
- Wendel J, Cronn R, Alvarez I, Liu B, Small R, Senchina D: Intron size and genome size in plants. Mol Biol Evol. 2002, 12: 2346-52.View ArticleGoogle Scholar
- Lanier W, Moustafa A, Bhattacharya D, Comeron J: EST analysis of Ostreococcus lucimarinus, the most compact eukaryotic genome, shows an excess of introns in highly expressed genes. PLoS One. 2008, 3 (5): e2171-10.1371/journal.pone.0002171.PubMedPubMed CentralView ArticleGoogle Scholar
- Wang J, Li S, Zhang Y, Zheng H, Xu Z, Ye J, Yu J, Wong G: Vertebrate gene predictions and the problem of large genes. Nat Rev Genet. 2003, 9: 741-9. 10.1038/nrg1160.View ArticleGoogle Scholar
- Coyne RS, Thiagarajan M, Jones KM, Wortman JR, Tallon LJ, Haas BJ, Cassidy-Hanley DM, Wiley EA, Smith JJ, Collins K, Lee SR, Couvillion MT, Liu Y, Garg J, Pearlman RE, Hamilton EP, Orias E, Eisen JA, Methé BA: Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure. BMC Genomics. 2008, 9: 562-10.1186/1471-2164-9-562.PubMedPubMed CentralView ArticleGoogle Scholar
- Huang Y, Niu D: Evidence against the energetic cost hypothesis for the short introns in highly expressed genes. BMC Evol Biol. 2008, 8: 154-10.1186/1471-2148-8-154.PubMedPubMed CentralView ArticleGoogle Scholar
- Ren XY, Vorst O, Fiers MW, Stiekema WJ, Nap JP: In plants, highly expressed genes are the least compact. Trends Genet. 2006, 22 (10): 528-32. 10.1016/j.tig.2006.08.008.PubMedView ArticleGoogle Scholar
- Wendel J, Cronn R, Johnston J, Price H: Feast and famine in plant genomes. Genetica. 2002, 115 (1): 37-47. 10.1023/A:1016020030189.PubMedView ArticleGoogle Scholar
- Vitte C, Panaud O, Quesneville H: LTR retrotransposons in rice (Oryza sativa, L.): recent burst amplifications followed by rapid DNA loss. BMC Genomics. 2007, 8: 218-10.1186/1471-2164-8-218.PubMedPubMed CentralView ArticleGoogle Scholar
- Wang H, Liu J: LTR retrotransposon landscape in Medicago truncatula: more rapid removal than in rice. BMC Genomics. 2008, 9: 382-10.1186/1471-2164-9-382.PubMedPubMed CentralView ArticleGoogle Scholar
- SanMiguel P, Tikhonov A, Jin YK, Motchoulskaia N, Zakharov D, Melake-Berhan A, Springer PS, Edwards KJ, Lee M, Avramova Z, Bennetzen JL: Nested retrotransposons in the intergenic regions of the maize genome. Science. 1996, 274 (5288): 765-8. 10.1126/science.274.5288.765.PubMedView ArticleGoogle Scholar
- Moisy C, Garrison K, Meredith C, Pelsy F: Characterization of ten novel Ty1/copia-like retrotransposon families of the grapevine genome. BMC Genomics. 2008, 9: 469-10.1186/1471-2164-9-469.PubMedPubMed CentralView ArticleGoogle Scholar
- Wawrzynski A, Ashfield T, Chen NW, Mammadov J, Nguyen A, Podicheti R, Cannon SB, Thareau V, Ameline-Torregrosa C, Cannon E, Chacko B, Couloux A, Dalwani A, Denny R, Deshpande S, Egan AN, Glover N, Howell S, Ilut D, Lai H, Del Campo SM, Metcalf M, O'Bleness M, Pfeil BE, Ratnaparkhe MB, Samain S, Sanders I, Ségurens B, Sévignac M, Sherman-Broyles S, Tucker DM, Yi J, Doyle JJ, Geffroy V, Roe BA, Maroof MA, Young ND, Innes RW: Replication of nonautonomous retroelements in soybean appears to be both recent and common. Plant Physiol. 2008, 148 (4): 1760-71. 10.1104/pp.108.127910.PubMedPubMed CentralView ArticleGoogle Scholar
- Vitte C, Panaud O: LTR retrotransposons and flowering plant genome size: emergence of the increase/decrease model. Genome Res. 2005, 110 (1-4): 91-107.View ArticleGoogle Scholar
- Wang H, Moore MJ, Soltis PS, Bell CD, Brockington SF, Alexandre R, Davis CC, Latvis M, Manchester SR, Soltis DE: Rosid radiation and the rapid rise of angiosperm-dominated forests. Proc Natl Acad Sci USA. 2009, 106 (10): 3853-8. 10.1073/pnas.0813376106.PubMedPubMed CentralView ArticleGoogle Scholar
- Wikström N, Savolainen V, Chase MW: Evolution of the angiosperms: calibrating the family tree. Proc Biol Sci. 2001, 268 (1482): 2211-20.PubMedPubMed CentralView ArticleGoogle Scholar
- Magallón S, Castillo A: Angiosperm diversification through time. American Journal of Botany. 2009, 96: 349-365.PubMedView ArticleGoogle Scholar
- Costa J, de Melo D, Gouveia Z, Cardoso H, Peixe A, Arnholdt-Schmitt B: The alternative oxidase family of Vitis vinifera reveals an attractive model to study the importance of genomic design. Physiol Plant. 2009, 137 (4): 553-65. 10.1111/j.1399-3054.2009.01267.x.PubMedView ArticleGoogle Scholar
- Tsukahara S, Kobayashi A, Kawabe A, Mathieu O, Miura A, Kakutani T: Bursts of retrotransposition reproduced in Arabidopsis. Nature. 2009, 461 (7262): 423-6. 10.1038/nature08351.PubMedView ArticleGoogle Scholar
- Wessler S, Bureau T, Whitea S: LTR-retrotransposons and MITEs: important players in the evolution of plant genomes. Curr Opin Genet Dev. 1995, 5 (6): 814-21. 10.1016/0959-437X(95)80016-X.PubMedView ArticleGoogle Scholar
- Hawkins J, Proulx S, Rapp R, Wendel J: Rapid DNA loss as a counterbalance to genome expansion through retrotransposon proliferation in plants. Proc Natl Acad Sci USA. 2009, 106 (42): 17811-6. 10.1073/pnas.0904339106.PubMedPubMed CentralView ArticleGoogle Scholar
- Barnaud A, Laucou V, This P, Lacombe T, Doligez A: Linkage disequilibrium in wild French grapevine, Vitis vinifera L. subsp. silvestris. Heredity. 2009,Google Scholar
- Baucom R, Estill J, Leebens-Mack J, Bennetzen J: Natural selection on gene function drives the evolution of LTR retrotransposon families in the rice genome. Genome Res. 2009, 19 (2): 243-54. 10.1101/gr.083360.108.PubMedPubMed CentralView ArticleGoogle Scholar
- Naito K, Cho E, Yang G, Campbell M, Yano K, Okumoto Y, Tanisaka T, Wessler S: Dramatic amplification of a rice transposable element during recent domestication. Proc Natl Acad Sci USA. 2006, 103 (47): 17620-5. 10.1073/pnas.0605421103.PubMedPubMed CentralView ArticleGoogle Scholar
- Gao L, McCarthy E, Ganko E, McDonald J: Evolutionary history of Oryza sativa LTR retrotransposons: a preliminary survey of the rice genome sequences. BMC Genomics. 2004, 5 (1): 18-10.1186/1471-2164-5-18.PubMedPubMed CentralView ArticleGoogle Scholar
- Parkinson J, Anthony A, Wasmuth J, Schmid R, Hedley A, Blaxter M: PartiGene--constructing partial genomes. Bioinformatics. 2004, 20 (9): 1398-404. 10.1093/bioinformatics/bth101.PubMedView ArticleGoogle Scholar
- Parkinson J, Guiliano D, Blaxter M: Making sense of EST sequences by CLOBBing them. BMC Bioinformatics. 2002, 3: 31-10.1186/1471-2105-3-31.PubMedPubMed CentralView ArticleGoogle Scholar
- Pertea G, Huang X, Liang F, Antonescu V, Sultana R, Karamycheva S, Lee Y, White J, Cheung F, Parvizi B, Tsai J, Quackenbush J: TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets. Bioinformatics. 2003, 19 (5): 651-2. 10.1093/bioinformatics/btg034.PubMedView ArticleGoogle Scholar
- Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W, Lipman D: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.PubMedPubMed CentralView ArticleGoogle Scholar
- de la Bastide M, McCombie W: Assembling genomic DNA sequences with PHRAP. Curr Protoc Bioinformatics. 2007, Chapter 11: Unit11.4-PubMedGoogle Scholar
- Iseli C, Jongeneel C, Bucher P: ESTScan: a program for detecting, evaluating, and reconstructing potential coding regions in EST sequences. Proc Int Conf Intell Syst Mol Biol. 1999, 1999: 138-48.Google Scholar
- Kent W: BLAT--the BLAST-like alignment tool. Genome Res. 2002, 12 (4): 656-64.PubMedPubMed CentralView ArticleGoogle Scholar
- Finn RD, Mistry J, Tate J, Coggill P, Heger A, Pollington JE, Gavin OL, Gunesekaran P, Ceric G, Forslund K, Holm L, Sonnhammer EL, Eddy SR, Bateman A: The Pfam protein families database. Nucleic Acids Research. 2010, D211-222. 10.1093/nar/gkp985. 38 Database
- Smit A, Hubley R, Green P: RepeatMasker. 2009, [http://repeatmasker.org]Google Scholar
- Xu Z, Wang H: LTR_FINDER: an efficient tool for the prediction of full-length LTR retrotransposons. Nucleic Acids Res. 2007, W265-8. 10.1093/nar/gkm286. 35 Web Server
- SanMiguel P, Gaut B, Tikhonov A, Nakajima Y, Bennetzen J: The paleontology of intergene retrotransposons of maize. Nature. 1998, 20: 43-45.Google Scholar
- Jukes T, Cantor C: Evolution of protein molecules. Edited by: Munro H. 1990, Mammalian protein metabolism. Academic press, 21-132.Google Scholar
- R Development Core Team: R: A language and environment for statistical computing. 2009, R Foundation for Statistical Computing, Vienna, Austria, ISBN 3-900051-07-0, [http://www.R-project.org]Google Scholar