Table 2 Primers used for confirmation of homozygous lines
From: Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbia
Allele | Primer sequence (5'-3′) |  |
|---|---|---|
CHS: tt4-13 | Intact Gene | GATCACTCATGTCGTCTTCTG |
(SALK_020583) | Â | AGGGCCAGGCGGTGAAG |
| Â | T-DNA insertion | GATCACTCATGTCGTCTTCTG |
| Â | Â | TTAGAGAGGAACGCTGTGC |
CHI: tt5-2 | Intact Gene | ATGTCTTCATCCAACGCCTG |
(CS300857) | Â | GTTCTCTTTGGCTAGTTTTTC |
| Â | T-DNA insertion | ATGTCTTCATCCAACGCCTG |
CHI: tt5-3 | Intact Gene | CGAAAGTAAGAATTAGAGAATAC |
(SALK_034145) | Â | AGGGCCAGGCGGTGAAG |
| Â | T-DNA insertion | CGAAAGTAAGAATTAGAGAATAC TGATAAACTTCTCAAACGCAC |
F3H: tt6-3 | Intact Gene | TGGTAGGTAGCTAGCGAC |
(SALK_113904) | Â | AACACACCGCGCCTAGC |
| Â | T-DNA insertion | TGGTAGGTAGCTAGCGAC |
| Â | Â | AGGGCCAGGCGGTGAAG |
F3′H: tt7-5 | Intact Gene | CAGCGGATTGGAATTTGAAC |
(SALK_053394) | Â | CAGCTGTGAACATGTTCTG |
| Â | T-DNA insertion | GGACCGCTTGCTGCAACT |
| Â | Â | CAGCTGTGAACATGTTCTG |
ANS: tt11-11 | Intact Gene | AGAGTTGAGAGTCTAGC |
(SALK_073183) | Â | GCAAAAGTCCGTGGAG |
| Â | T-DNA insertion | AGAGTTGAGAGTCTAGC |
| Â | Â | TGGTTCACGTAGTGGGCCATCG |
ANR: ban-4 | Intact Gene | TGGACCAGACTCTTAC |
(SALK_040250) | Â | AGACCGGTCACATGC |
| Â | T-DNA insertion | AGACCGGTCACATGC |
| Â | Â | TGGTTCACGTAGTGGGCCATCG |