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Figure 2 | BMC Research Notes

Figure 2

From: Development of a broad-host-range sacB-based vector for unmarked allelic exchange

Figure 2

Broad-host-range allelic exchange vector pCM433. Plasmid map of pCM433 [GenBank:EU118176] indicating the available restriction sites in the multiple-cloning site, as well as key plasmid features: bla (encodes betalactamase; ApR), oriT (IncP origin of conjugal transfer), tet (encodes Tc efflux pump), cat (encodes Cm actetyltransferase), sacB (levansucrase; confers sucrose sensitivity), and ColE1 ori (high copy E. coli origin of replication). Plasmid map was generated using Clone Manager 7 (Sci Ed Central).

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