Figure 5

C19ORF5 or MAP1B depletion does not interfere with NORE1A-induced growth suppression. A549 cells were transfected with anti-MAP1B siRNA pool (A) or control siRNA, or anti-C19ORF5 siRNA pool or control siRNA (B) then infected with retrovirus expressing GFP or GFP-NORE1A effector domain (aa 191–363). Two days later, cells were fixed, processed for flow cytometry and analyzed as described in Methods. The percentage of GFP-positive cells in each phase of the cell cycle +/- mean is shown.