Figure 6

Functionality of GFP gene from phRGFP plasmid is preserved in the products of recombination with pINS-Puro, pINS-Neo or pINS-Blast plasmids. HeLa cells transfected by 1 mkg of either Target vector phrGFP or the products of recombination (Blast × GFP, Neo × GFP and Puro × GFP). Cells transfected by the recombination products were selected by either blasticidin S, G418 or puromycin and stained by DAPI. Expression of the GFP was analyzed under the microscope. In case of transient transfection by phrGFP vector we usually observed 25% GFP positive cells. In contrast we observed that 80–100% of the cells transfected by the products of recombination and selected by the corresponding antibiotics are GFP positive. Scale bar: 20 μm.