Skip to main content

Table 2 Summary of the clinical characteristics, RNA quality and cDNA preamplification results for the 22 samples analyzed in this study.

From: Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays

Sample number Type of sample Type of bladder fluid Tumor characteristics RNA characteristics N° validated genes (CT ≤ 31) Correlation coeficients (r) N° validated genes in NPA samples with ΔΔCT outside ± 1.5 Gene symbol
      rRNA ratio RIN % of total      
    stage grade    18S area 28S area NPA samples PA samples    
1 T BW Ta LG 2 9.8 20.6 40.4 33 43 0.986 1 IGF2
2 T BW Ta LG 1.7 9.2 18.5 31.6 39 46 0.978 4 CRH, FGFR3, IGF2,KRT14
3 T BW Ta LG 1.7 8.9 18.5 32.1 38 45 0.987 2 CRH,IGF2
4 T BW Ta LG 1.6 8.8 17.0 26.8 36 45 0.991 1 IGF2
5 T BW Ta LG 1.3 8.2 14.2 18.1 25 42 0.987 1 IGF2
6 T BW T2b+CIS HG 1.8 8 9.3 16.7 38 44 0.967 3 CCNA2, IGF2, MAGEA9
7 T BW T1 HG 2.3 7.8 8.1 18.7 35 43 0.975 3 FGFR3, IGF2, MELK
8 C BW - - 1.5 7.6 8.6 12.6 19 40 0.985 0 -
9 T BW T1c HG 1.6 7.5 9.4 15.0 32 42 0.98 5 FGFR3, IGF2, KIF2C, MELK, PDZRN3
10 T U T1c+CIS HG 1.1 7.5 13.6 15.0 40 45 0.961 2 CRH, IGF2
11 T U T1b HG 0.8 7.5 12.8 10.1 41 46 0.956 3 CRH, FGFR3, IGF2
12 T U T1 HG 1.2 7.4 10.5 12.3 39 45 0.972 2 CRH, IGF2
13 C BW - - 1.3 7.2 7.9 10.0 28 39 0.976 1 IGF2
14 T BW T1+CIS HG 1.3 6.4 6.4 8.4 35 43 0.975 3 CRH, FGFR3, IGF2
15 C BW - - 0.8 2.8 1,6 1,3 3 13 0.937 0 _
16 C U - - 0 2.5 N/A N/A 8 14 0.95 1 KRT14
17 T U T2 HG 0 2.5 N/A N/A 11 39 0.994 1 CDC2
18 T U T1 HG 0 2.4 N/A N/A 13 41 0.965 3 GJB2, FGFR3, IGF2
19 T U T2+CIS HG 0 2.4 N/A N/A 27 40 0.97 5 CCNA2, CRH, FGFR3, IGF2, KRT14
20 T U T2+CIS HG 0 2.3 N/A N/A 31 45 0.938 2 IGF2, TOP2A
21 C BW - - 1.3 N/A 3.8 4.6 18 36 0.943 2 IGF2, FGFR3
22 T BW T2b HG 1.1 N/A 8.6 9.4 40 45 0.968 1 IGF2
  1. Legend/key: Samples are ranked by RIN number. BW, Bladder Washing; CIS, Carcinoma In Situ; C, Control; CT, Cycle Threshold; HG, High Grade; LG, Low Grade; N/A, Non Available; NPA, Non-Preamplified; PA, Preamplified; RIN, RNA Integrity Number; T, Tumor; U, Urine.