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Table 3 Number of validated genes of two serial dilutions of 3 cDNA samples before and after their preamplification.

From: Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays

  Initial cDNA
  25 ng of preamplified cDNA
Sample number N° validated genes (CT ≤ 31) N° validated genes with ΔΔCT outside ± 1.5 r
  NPA samples PA samples   
2 39 46 4 0.978
11 41 46 3 0.956
22 40 45 1 0.968
ng cDNA/port 500 15000   
Dilution 1/20 of the initial cDNA
  1.25 ng of preamplified cDNA
Sample number N° validated genes (CT ≤ 31) N° validated genes with ΔΔCT outside ± 1.5 r
  NPA samples PA samples   
2 14 38 6 0.972
11 22 40 2 0.967
22 6 39 5 0.988
ng cDNA/port 25 750   
Dilution 1/400 of the initial cDNA
  0.06 ng of preamplified cDNA
Sample number N° validated genes (CT ≤ 31) N° validated genes with ΔΔCT outside ± 1.5 r
  NPA samples PA samples   
2 3 17 0 0.962
11 2 27 3 0.883
22 3 20 2 0.932
ng cDNA/port 1.25 37.5   
  1. Legend/key: The number of each sample corresponds to the "sample number" indicated in Table 2. CT, Cycle Threshold; NPA, Non-Preamplified; PA, Preamplified.