Figure 3

ASMD sequence comparison display. This screen capture shows the sequence comparison display from the detailed mutation view (obtained by clicking on the ASMD ID in the search results). The mutation position is highlighted in red. The splice site strength scores are shown in blue along with the flanking intronic sequences. The putative local RNA secondary structure ("fold") is shown along with its minimum free energy (mfe) value. Note how the mutation disrupts the base-pairing in the stem of the fold, substantially reducing the strength of the fold.