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Table 2 Microarray identified genes in pneumococcal strain TIGR4 upon exposure to A549 cells for 0.5 h and 1 h time periods

From: Streptococcus pneumoniaeearly response genes to human lung epithelial cells

Function/gene name Protein TIGR4 genome acc. No. Incubation time
    0.5 h 1 h
Cell envelope
cbpI a choline binding protein I SP0069   2.8
cps4A capsular polysaccharide biosynthesis protein Cps4A SP0346   2.9
cps4B capsular polysaccharide biosynthesis protein Cps4B SP0347   2.0
cps4C capsular polysaccharide biosynthesis protein Cps4C SP0348   3.3
cps4E capsular polysaccharide biosynthesis protein Cps4E SP0350   2.9
cps4I UDP-N-acetylglucosamine-2-epimerase SP0357   2.4
bgaA β-galactosidase SP0648   17.0
nanA a neuraminidase A, authentic frameshift SP1693   16.5
Energy metabolism
agaS sugar isomerase domain protein AgaS SP0065   5.6
pyk pyruvate kinase SP0897 -2.7  
glgA glycogen synthase SP1124   3.8
  acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase, putative SP1162   2.7
zwf glucose-6-phosphate 1-dehydrogenase SP1243 -2.7  
scrB sucrose-6-phosphate hydrolase SP1724 3.0 4.4
galT galactose-1-phosphate uridylyltransferase SP1852   2.7
galK galactokinase SP1853   2.3
recP transketolase SP2030   -3.6
arcA arginine deiminase SP2148 4.6  
gplK glycerol kinase SP2186   3.0
Hypothetical proteins
  conserved hypothetical protein SP0024   -2.6
  hypothetical protein SP0026 -2.3  
  hypothetical protein SP0052 -3.5 -5.6
  hypothetical protein SP0067 2.4 2.1
  conserved hypothetical protein SP0095 -2.4  
  conserved hypothetical protein SP0159 -2.3  
  hypothetical protein SP0190 2.3  
  hypothetical protein SP0203 -2.5  
  conserved hypothetical protein SP0207 -2.1  
  conserved hypothetical protein SP0288 -4.2 -2.2
  conserved hypothetical protein SP0742   -2.9
  conserved hypothetical protein SP0951   2.4
  conserved hypothetical protein SP1003   2.1
  hypothetical protein SP1049 2.0  
  hypothetical protein SP1059   4.4
  conserved hypothetical protein SP1174   2.4
  hypothetical protein SP1198 2.7 2.6
  hypothetical protein SP1199 2.9 2.0
  conserved hypothetical protein SP1601   2.4
  hypothetical protein SP1677   10.3
  hypothetical protein SP1678 2.9 6.1
  hypothetical protein SP1679 4.6 9.6
  conserved hypothetical protein SP1680 5.3 11.5
  hypothetical protein SP2183 2.7 4.1
Others
  bacteriocin, putative SP0109 2.3  
  lactose phosphotransferase system repressor, degenerate SP0169   2.2
  dihydropteroate synthase SP0289 -2.2 -2.1
acpP acyl carrier protein SP0418 -2.0  
fabF 3-oxoacyl-(acyl-carrier-protein) synthase II SP0422 -2.4  
accD acetyl-CoA carboxylase, carboxyl transferase subunit beta SP0426 -2.4  
accA acetyl-CoA carboxylase, carboxyl transferase subunit alpha SP0427 -3.4  
ilvB acetolactate synthase, large subunit, biosynthetic type SP0445 -2.8  
zmpB zinc metalloprotease ZmpB SP0664 -2.1  
ilvE branched-chain amino acid aminotransferase SP0856 -2.0  
  preprotein translocase, SecG subunit, putative SP0974   2.5
asd aspartate-semialdehyde dehydrogenase SP1013 -2.0  
bta bacterocin transport accessory protein SP1499 -2.7 -2.4
  transcriptional regulator, MerR family SP1856 2.0  
groEL chaperonin, 60 kDa SP1906   -2.4
Protein synthesis
rpsD ribosomal protein S4 SP0085   2.7
rpsJ ribosomal protein S10 SP0208   4.1
rplW ribosomal protein L23 SP0211   2.9
rpsC ribosomal protein S3 SP0215   2.0
infA translation initiation factor IF-1 SP0232   2.4
valS valyl-tRNA synthetase SP0568 -2.1  
rplK ribosomal protein L11 SP0630   2.5
infC translation initiation factor IF-3 SP0959   2.5
rpml ribosomal protein L35 SP0960   3.9
rpsR ribosomal protein S18 SP1539   2.8
rpsF ribosomal protein S6 SP1541 2.9 3.0
rpmH ribosomal protein L34 SP1993   2.4
rpmG ribosomal protein L33 SP2135   2.1
yfiA ribosomal subunit interface protein SP2206   -3.9
Purine and pyrimidine ribonucleotide biosynthesis
purA adenylosuccinate synthetase SP0019 -2.5  
purC a phosphoribosylaminoimidazole-succinocarboxamide synthase SP0044 -5.1 -4.7
purH phosphoribosylaminoimidazolecarboxamide formyltransferase-IMP cyclohydrolase SP0050 -15.3 -4.1
purE a phosphoribosylaminoimidazole carboxylase, catalytic subunit SP0053 -6.4 -8.5
purK a phosphoribosylaminoimidazole carboxylase, ATPase subunit SP0054   -2.4
nrdD anaerobic ribonucleoside-triphosphate reductase SP0202 -4.4 -4.3
nrdG anaerobic ribonucleoside-triphosphate reductase activating protein SP0205 -3.4 -2.8
thyA thymidylate synthase SP0669 -2.2  
pyrK dihydroorotate dehydrogenase, electron transfer subunit SP0963   -3.6
nrdH NrdH-redoxin SP1178   -2.1
carB carbamoyl-phosphate synthase, large subunit SP1275   -4.2
pyrR pyrimidine operon regulatory protein SP1278 -2.1 -7.8
guaA a GMP synthase SP1445 -2.4  
purR pur operon repressor SP1979 -2.7 -2.3
Transport
  PTS system, IIA component SP0064 2.2 3.4
  PTS system, mannose-specific IID component SP0282 -3.7  
  xanthine-uracil permease family protein SP0287 -8.4 -5.3
  O-antigen transporter RfbX, putative SP0356   2.5
  PTS system, IIC component, putative SP0647   4.3
  sugar ABC transporter, ATP-binding protein SP0846 -2.1  
  ABC transporter, permease protein SP1688   5.3
  ABC transporter, permease protein SP1689   2.8
  ABC transporter, substrate-binding protein SP1690   2.1
msmE sugar ABC transporter, sugar-binding protein SP1897   2.1
malD maltodextrin ABC transporter, permease protein SP2110   2.6
Unknown function
  vanZ protein, putative SP0049 -2.9  
  ACT domain protein SP0238 -2.1 -3.4
  HIT family protein SP0521   -2.4
gid Gid protein SP0943   -2.2
  flavoprotein SP1231   -2.0
usp45 secreted 45 kd protein SP2216 2.1  
  1. a genes that are also involved in pathogenesis according to TIGR genome annotation