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Figure 3 | BMC Research Notes

Figure 3

From: PeakSeeker: a program for interpreting genotypes of mononucleotide repeats

Figure 3

Typical data analysis and output of PeakSeeker program. A. Experimental electropherogram for locus 502. Signal intensity is displayed on the Y-axis (in arbitrary units), signal size (bp) is on the X-axis. B. Plot of weighted RMSD score (X-axis) versus score for likelihood of unequal allelic amplification (Y-axis) for all electropherogram interpretations considered in this example. Red arrow indicates a single interpretation that is maximal along both axes. C. In ascending order, panels represent the four most likely interpretations of the experimental genotype as the homozygous or heterozygous combination of two alleles. The experimental genotype is displayed in blue, with electropherogram peaks represented at the intersection of line segments (open circles). Inferred genotypes of the individual alleles are represented by black traces, and the peaks of their additive product are displayed in red (open triangles). The relative amplification of the two alleles (K1 and K2) is displayed at the bottom of each panel, with the lengths of the proposed alleles reported at the top of each panel, below which is the adjusted RMSD score for the proposed genotype interpretation. The correct call, with the lowest score, is indicated in red, and corresponds to the score indicated in panel B.

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