Figure 1

Mid-gestation inactivation of BUB1 arrests embryonic development. (A) Schematic of mating regimen. A BUB1^F/FER^T-Cre male was crossed with a BUB1^Δ/+ ER^T-Cre female. The pregnant female was injected with tamoxifen at 10.5 dpc, then the embryos were harvested and genotyped at various times after injection. (B) PCR genotyping of 12.5-dpc embryos to determine the BUB1 genotype (top and middle panels) and to detect the presence of Cre (lower panel). See main text for details. (C) Images of BUB1^Δ/+ embryos (panels i to iv) and BUB1^Δ/Δ embryos (panels v to viii) after harvesting on the indicated day post coitum, after removal of the placenta and yolk sac. Scale bar, 5 mm.