SDH and FH MS-HRM in breast cancer cell lines. In MS-HRM, PCR products undergo melting analysis after PCR amplification. Different amplicons have different melting behaviours according to their methylation status. Unmethylated samples melt earlier than methylated samples as they have unmethylated cytosines replaced by thymines in the sequence. Controls for 100%, 25%, 5% methylation and WGA are shown. Levels of methylation as low as 5% can be readily seen. The cell lines shown here, MCF7 and T47D showed no methylation of the four SDH subunits and FH. A. SDHA; B. SDHB; C. SDHC; D. SDHD; and E. FH methylation. The curve for each sample represents the mean value of duplicate samples.