Table 1 Primer sequences, annealing temperature and amplicon information for the MS-HRM assays.
Gene | Primer Sequences 5' - 3' | Annealing temperature (°C) | Amplified region (GenBank accession and nucleotide numbers) | Screened CpGs/amplicon size (bp) |
|---|---|---|---|---|
SDHA | F - CGGGGTTTTAAAAATGTTGGTGTT | 61 | AC021087.5: 218153-218484 | 39/332 |
R - CGAACCCCCGACATATCTACTATTACC | ||||
SDHB 1 | F - CGGGGGAAGTTAAATGGGTATG | 60 | AL049569.13: 17380588-17380744 | 14/157 |
R - CGCCCAACCTACATCCACTAAA | ||||
SDHB 2 | F - GCGGTTAGTGGGTTTTTAGTGGAT | 65 | AL049569.13: 17380446-17380623 | 16/178 |
R - CAAACAAACTCCGCCAAAAATTATAACC | ||||
SDHC | F - TCGTTATATGATATTTTTAATTTCGATTTTTAGT | 56 | AL592295.25: 161284096-161284197 | 8/102 |
R - ATCTTAAATTCCGATCTAAACGAAAATAAC | ||||
SDHD | F - CGGGTTGGTGGATGATTTTGAG | 62 | AP002007.4: 111957596-111957689 | 4/94 |
R - CCTCACCTCGACCTCCTAAAACAC | ||||
FH | F - TTTGTTTTATTTGTCGGTGTGAGGT | 60 | AL591898.1: 241683032-241683154 | 7/123 |
R - AAAACTTAAATAAAATTTCTAAACGACTATAACCAC |