Extent of epiboly at 8 hpf
|
MO3 alone
|
MO3 + mRNA
|
---|
|
% (n)
|
% (n)
|
---|
75% epiboly - bud
|
4.8 (7)
|
21 (30)
|
50-75% epiboly
|
49.0 (71)
|
52.4 (75)
|
30-50% epiboly
|
46.2 (67)
|
26.6 (38)
|
Total number injected (n)
|
(145)
|
(143)
|
- MO3 at 1 mM was injected into the embryos at the 1-cell stage. aplnra mRNA at 100 ng/μL was injected into the yolk cell immediately vegetal to the embryo at the 32-cell stage. A contingency χ2 test of the data across all the phenotypic classes gives p < 0.001.