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Table 1 Average germination and sterility rates (%) of M1 plants observed after different EMS Treatments

From: TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1

  Fd) EMS treatments
   0 mM 20 mM 25 mM 30 mM 35 mM 40 mM 45 mM 50 mM 55 mM 60 mM
germinationb) 6.1** - 92 89 81 75.3 69.3 59.7 56.7 49 40.5
full sterilityc) 41.7*** 4 3 21 26 30 63 84 87.7 87.5 95.5
semi-sterilityc) 17.5*** 63 81 74 83 68 37 16 13 12 5
no sterilityc) 8.97*** 33 16 5 2 1.8 0.6 0.06 0 0 0
  1. a) In total 72,879 M1 plants were cultivated in 299-well seed trays on flood tables.
  2. b) All germination data were collected as percentages of germination rates observed for the 0 mM control plants.
  3. c) Sterility was scored for the examined M1 spikes in three categories: fully sterile (100%), semi-sterile (measured as > 50%, 50%, < 50%), or no sterility (0%).
  4. d) The one factorial analysis of variance (ANOVA) tested the variability within each EMS concentration and between all concentrations. ANOVA: F = Fisher's exact test: *: Significance at p ≤ 0.05; ** Significance at p ≤ 0.01; *** Significance at p ≤ 0.001. A test of linearity was performed to confirm the required normality of the single data set (data not shown) [63].
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