| Â | Fd) | EMS treatments |
|---|
| Â | Â | 0 mM | 20 mM | 25 mM | 30 mM | 35 mM | 40 mM | 45 mM | 50 mM | 55 mM | 60 mM |
|---|
germinationb) | 6.1** | - | 92 | 89 | 81 | 75.3 | 69.3 | 59.7 | 56.7 | 49 | 40.5 |
full sterilityc) | 41.7*** | 4 | 3 | 21 | 26 | 30 | 63 | 84 | 87.7 | 87.5 | 95.5 |
semi-sterilityc) | 17.5*** | 63 | 81 | 74 | 83 | 68 | 37 | 16 | 13 | 12 | 5 |
no sterilityc) | 8.97*** | 33 | 16 | 5 | 2 | 1.8 | 0.6 | 0.06 | 0 | 0 | 0 |
- a) In total 72,879 M1 plants were cultivated in 299-well seed trays on flood tables.
- b) All germination data were collected as percentages of germination rates observed for the 0 mM control plants.
- c) Sterility was scored for the examined M1 spikes in three categories: fully sterile (100%), semi-sterile (measured as > 50%, 50%, < 50%), or no sterility (0%).
- d) The one factorial analysis of variance (ANOVA) tested the variability within each EMS concentration and between all concentrations. ANOVA: F = Fisher's exact test: *: Significance at p ≤ 0.05; ** Significance at p ≤ 0.01; *** Significance at p ≤ 0.001. A test of linearity was performed to confirm the required normality of the single data set (data not shown) [63].
