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Table 4 List of TILLING targets, size of amplicons, and the number and distribution of mutation types

From: TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1

Gene Description Amplicon Amplicon
size (bp)1)
Mutation Frequency (Mb)5)
     total Intron syn2) non-syn3) trun4)  
HvCO1 6) CONSTANS-like gene 1 830 8 - 5 3 - 1/0.67
Mlo9 7) Modulator of powdery mildew resistance and cell death 1 900 2 - - 1 1 1/0.75
   2 1476 3 1 2 - - 1/0.87
HveIF4E 8) eukaryotic translation
initiation factor 4E
1 367 7 3 - 4 - 1/0.28
   2 321 4 2 - 2 - 1/0.41
HvDnaJ-like 9) DnaJ-like chaperone 1 434 7 2 2 3 - 1/0.36
HvHox1 10) Homeodomain transcription
factor controlling row-type morphology in barley
1 985 22 7 6 8 1 1/0.3
   2 362 9 2 5 2 - 1/0.2
HvCIGR2 11) Barley homolog of rice
'Chitin-inducible
gibberellin-responsive'
1 699 13 - 10 2 1 1/0.34
   2 702 6 - 2 4 - 1/0.63
  1. 1) 7,389 M2 lines were screened for mutations in the listed genes, except for the Mlo9 gene that was analysed in 1,920 M2 lines. 2) syn = synonymous mutations that do not alter the AA sequence of the protein; 3) non-syn = non-synonymous mutations that introduce a change of AA in the protein sequence; 4) trun = truncation mutations that generate a premature stop codon or alter a splice junction, potentially resulting in a truncation of the protein; 5) The mutation frequency for each amplicon is calculated as follows: (size of amplicon --100 bp × total number of screened samples)/total number of identified mutations; 100 bp were subtracted because of the diminished ability to detect mutations in the upper and lower 50 bp; 6) [64]7) [65, 66]8) [56]9) NCBI accession BQ470183 10) [15]11) NCBI accession EU914128 (bp position 69,100-70,440)
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