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Table 2 Descriptive statistics of the tested reference genes

From: Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis

  Rps18 Sdha Canx Pgk1 Actg1 Ubc Rpl13a Actb
GenBank ID GU188049 GU188053 GU188051 GU188052 GU188050 GU188054 GU188056 GU188055
AM amplification rate 1.86 1.88 1.83 1.85 1.88 1.86 1.85 1.82
CV amplification rate 0.06 0.05 0.05 0.05 0.06 0.05 0.05 0.05
GM Ct-value 14.62 15.37 16.63 15.72 16.83 15.72 26.05 16.05
AM Ct-value 14.64 15.42 16.68 15.78 16.88 15.79 26.10 16.16
CV Ct-value 0.04 0.07 0.07 0.07 0.07 0.08 0.06 0.09
Minimum Ct-value 13.86 13.90 14.65 13.42 14.94 12.87 24.30 13.64
Maximum Ct-value 16.74 17.68 19.61 18.62 19.68 18.26 28.87 20.50
SD Ct-value 0.65 1.09 1.09 1.10 1.11 1.26 1.45 1.49
SD-threshold 1.12 1.10 1.15 1.13 1.10 1.12 1.13 1.16
Minimum [x-fold] -1.60 -2.52 -3.30 -4.10 -3.29 -5.90 -2.94 -4.24
Maximum [x-fold] 3.71 4.27 6.10 5.93 6.00 4.84 5.65 14.49
SD [± x-fold] 1.50 1.99 1.99 2.00 2.01 2.21 2.49 2.56
  1. Arithmetic mean (AM), geometric mean (GM), coefficient of variance (CV) and standard deviation (SD) of the amplification rate E and the Ct-values for every potential reference gene. The genes are ordered by their SDCt-value. Genes that showed a SDCt-valuesmaller than the SD-threshold are considered to be suitable reference genes and marked in bold. The last three rows show the maximum and minimum values of the over- and under-expression of a gene in relation to its calculated geometric mean (displayed as x-fold ratio) as well as the standard deviation (calculated with BestKeeper).