| |
Rps18
|
Sdha
|
Canx
|
Pgk1
|
Actg1
|
Ubc
|
Rpl13a
|
Actb
|
|---|
|
GenBank ID
|
GU188049
|
GU188053
|
GU188051
|
GU188052
|
GU188050
|
GU188054
|
GU188056
|
GU188055
|
|
AM
amplification rate
|
1.86
|
1.88
|
1.83
|
1.85
|
1.88
|
1.86
|
1.85
|
1.82
|
|
CV
amplification rate
|
0.06
|
0.05
|
0.05
|
0.05
|
0.06
|
0.05
|
0.05
|
0.05
|
|
GM
Ct-value
|
14.62
|
15.37
|
16.63
|
15.72
|
16.83
|
15.72
|
26.05
|
16.05
|
|
AM
Ct-value
|
14.64
|
15.42
|
16.68
|
15.78
|
16.88
|
15.79
|
26.10
|
16.16
|
|
CV
Ct-value
|
0.04
|
0.07
|
0.07
|
0.07
|
0.07
|
0.08
|
0.06
|
0.09
|
|
Minimum
Ct-value
|
13.86
|
13.90
|
14.65
|
13.42
|
14.94
|
12.87
|
24.30
|
13.64
|
|
Maximum
Ct-value
|
16.74
|
17.68
|
19.61
|
18.62
|
19.68
|
18.26
|
28.87
|
20.50
|
|
SD
Ct-value
|
0.65
|
1.09
|
1.09
|
1.10
|
1.11
|
1.26
|
1.45
|
1.49
|
|
SD-threshold
|
1.12
|
1.10
|
1.15
|
1.13
|
1.10
|
1.12
|
1.13
|
1.16
|
|
Minimum [x-fold]
|
-1.60
|
-2.52
|
-3.30
|
-4.10
|
-3.29
|
-5.90
|
-2.94
|
-4.24
|
|
Maximum [x-fold]
|
3.71
|
4.27
|
6.10
|
5.93
|
6.00
|
4.84
|
5.65
|
14.49
|
|
SD [± x-fold]
|
1.50
|
1.99
|
1.99
|
2.00
|
2.01
|
2.21
|
2.49
|
2.56
|
- Arithmetic mean (AM), geometric mean (GM), coefficient of variance (CV) and standard deviation (SD) of the amplification rate E and the Ct-values for every potential reference gene. The genes are ordered by their SDCt-value. Genes that showed a SDCt-valuesmaller than the SD-threshold are considered to be suitable reference genes and marked in bold. The last three rows show the maximum and minimum values of the over- and under-expression of a gene in relation to its calculated geometric mean (displayed as x-fold ratio) as well as the standard deviation (calculated with BestKeeper).
