Figure 1

Afferent activity to necklace glomeruli is dependent on exogenous stimulation of GC-D+ neurons. (A) RT-PCR of GC-D, PDE2 and uroguanylin from nasal epithelium cDNA of C57BL/6J mice with or without reverse transcriptase (RT). The identity of each product was confirmed by sequencing. (B) Immunohistochemical staining of horizontal sections of left (non-occluded side) and right (occluded side) MOBs of a Gucy2d-Mapt-lacZ ^+/- mouse. TH, green; DAPI, blue. Scale bar: 100 μM. (C) Immunohistochemical staining of left (non-occluded side) MOB of a naris occluded Gucy2d-Mapt-lacZ ^+/- mouse. Inset, higher magnification view of area represented in box. β-gal, red; TH, green; DAPI, blue. Scale bars 50 μM, 20 μM (inset). (D) Right (occluded side) MOB of same mouse as in (C). Inset, higher magnification view of area represented in box. β-gal, red; TH, green; DAPI, blue. Scale bars 50 μM, 20 μM (inset).