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Figure 2 | BMC Research Notes

Figure 2

From: Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature

Figure 2

Locus representation analysis of MDA using quantitative PCR (qPCR). Four new MDA primers (NNNNN*N*N, GGNN*N*N, AGGG*A*G and GGGCAGGA*N*G) in addition to the Repli-g kit primer (NNNN*N*N), were utilized in independent amplification reactions to amplify 50 ng of gDNA templates. 100% locus representation indicates complete specificity in the MDA reaction.

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