Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature

Table 1 Affymetrix 10K Xba142 DNA chip SNP call analysis

Sample	F-G	M-G	S-G	D-G	Average
% SNP Call	93.4	97.2	94.7	95.2	95.1
Sample	F-K30	M-K30	S-K30	D-K30	Average
% SNP Call	96.3	97.7	96.5	95.5	96.5
Sample	F-K40	M-K40	S-K40	D-K40	Average
% SNP Call	97.5	95.1	96.8	95.6	96.3
Sample	F-W30	M-W30	S-W30	D-W30	Average
% SNP Call	97.7	97.7	94.7	97.4	96.9
Sample	F-W40	M-W40	S-W40	D-W40	Average
% SNP Call	95.5	95.8	96.4	92.5	95.0

Percent call rates for gDNA and MDA samples are shown. Samples belong to one family including: Father (F), Mother (M), Son (S), and Daughter (D). G represents unamplified gDNA. K30 and K40 represent MDA reactions using Repli-g kit at 30°C and 40°C, respectively); W30 and W40 represent MDA reactions using GGGCAGGA*N*G primer at 30°C and 40°C, respectively.

ISSN: 1756-0500