Figure 5

GFP and DsRED reporter expression in the same fly dying from dessication/starvation. Two male flies were placed in an empty vial and tracked for 48 hours. Black/white bars indicate the light/dark cycle the flies were cultured under prior to the beginning of the experiment. Activity is expressed as distance moved per hour (cm/hr, indicated in blue) and fluorescence intensity is expressed as average pixel intensity per hour: GFP fluorescence is plotted in green and DsRED fluorescence is plotted in red. Arrows indicate the last time point at which the animals displayed spontaneous movement; these animals were scored as dead at the end of the experiment based on lack of movement in response to stimulation. One fly contained both the hsp70-GFP and hsp22-DsRED transgenes, genotype w[1118]; p{hsp22-DsRED} [1MI1]/CyO; p{hsp70-GFP} [1MI2]/TM3 Sb (A). The second fly contained both the hsp22-GFP and hsp70-DsRED transgenes, genotype w[1118]; p{hsp70-DsRED} [1MI2]/CyO; p{hsp22-GFP} [1MI1]/TM3 Sb (B).