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Table 4 Expected and observed concentrations for Rosetta spike-ins 11 and 12, including %CV and %Bias.

From: Multiplexed measurements of gene signatures in different analytes using the Nanostring nCounter™ Assay System

Rosetta spike-in #11
Transcript name Intended conc, AU Best Fit Expected Observed StdDev CV, % Bias, %
r60_a20:50:rp 100 32.09 28.62 1.47 5.2 10.8
r60_a22_rp 10 3.21 4.71 0.28 5.9 46.9
r60_a104_rp 10 3.21 2.62 0.14 5.2 18.5
r60_1_rp 10 3.21 4.29 0.26 6.1 33.6
r60_a107_rp 30 9.63 10.56 0.70 6.6 9.7
r60_3_rp 3 0.96 1.02 0.09 9.1 6.1
r60_a135_rp 9 2.89 3.13 0.16 5.0 8.3
r60_a97_rp 0.5 0.16 0.14 0.03 21.8 15.5
r60_n11:30:rp 1.5 0.48 0.34 0.07 21.3 30.1
Rosetta spike-in #12
r60_a20:50:rp 100 44.18 37.56 2.25 6.0 15.0
r60_a22_rp 100 44.18 81.54 5.51 6.8 84.5
r60_a104_rp 30 13.25 12.16 0.70 5.8 8.2
r60_1_rp 10 4.42 5.85 0.30 5.1 32.3
r60_a107_rp 10 4.42 4.49 0.36 8.0 1.6
r60_3_rp 9 3.98 4.28 0.25 5.8 7.7
r60_a135_rp 3 1.33 1.30 0.08 6.2 1.6
r60_a97_rp 1.5 0.66 0.61 0.07 11.3 7.9
r60_n11:30:rp 0.5 0.22 0.12 0.04 29.7 43.6
  1. Calculations based on a per-sample basis.
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