Table 4 Expected and observed concentrations for Rosetta spike-ins 11 and 12, including %CV and %Bias.
Rosetta spike-in #11 | ||||||
|---|---|---|---|---|---|---|
Transcript name | Intended conc, AU | Best Fit Expected | Observed | StdDev | CV, % | Bias, % |
r60_a20:50:rp | 100 | 32.09 | 28.62 | 1.47 | 5.2 | 10.8 |
r60_a22_rp | 10 | 3.21 | 4.71 | 0.28 | 5.9 | 46.9 |
r60_a104_rp | 10 | 3.21 | 2.62 | 0.14 | 5.2 | 18.5 |
r60_1_rp | 10 | 3.21 | 4.29 | 0.26 | 6.1 | 33.6 |
r60_a107_rp | 30 | 9.63 | 10.56 | 0.70 | 6.6 | 9.7 |
r60_3_rp | 3 | 0.96 | 1.02 | 0.09 | 9.1 | 6.1 |
r60_a135_rp | 9 | 2.89 | 3.13 | 0.16 | 5.0 | 8.3 |
r60_a97_rp | 0.5 | 0.16 | 0.14 | 0.03 | 21.8 | 15.5 |
r60_n11:30:rp | 1.5 | 0.48 | 0.34 | 0.07 | 21.3 | 30.1 |
Rosetta spike-in #12 | ||||||
r60_a20:50:rp | 100 | 44.18 | 37.56 | 2.25 | 6.0 | 15.0 |
r60_a22_rp | 100 | 44.18 | 81.54 | 5.51 | 6.8 | 84.5 |
r60_a104_rp | 30 | 13.25 | 12.16 | 0.70 | 5.8 | 8.2 |
r60_1_rp | 10 | 4.42 | 5.85 | 0.30 | 5.1 | 32.3 |
r60_a107_rp | 10 | 4.42 | 4.49 | 0.36 | 8.0 | 1.6 |
r60_3_rp | 9 | 3.98 | 4.28 | 0.25 | 5.8 | 7.7 |
r60_a135_rp | 3 | 1.33 | 1.30 | 0.08 | 6.2 | 1.6 |
r60_a97_rp | 1.5 | 0.66 | 0.61 | 0.07 | 11.3 | 7.9 |
r60_n11:30:rp | 0.5 | 0.22 | 0.12 | 0.04 | 29.7 | 43.6 |