Consistency and discrepancy between the RGS8 MLPA results and PCR deletion mapping of the same region. Each column represents the rate of copy number variation based upon MLPA analysis of 11 tumor samples, in which RGS8 intragenic deletions previously have been established by LOH analysis followed by PCR mapping. For each of the three fragments 14, 31, and 32 agreements between the two methods are illustrated by the rate of deletion and amplification obtained by MLPA for individual samples. Cases with no CNV and agreement between the two methods comprise 3 (fr. 14), 1 (fr. 31) and 4 (fr. 32) samples, are not included in figure. The 95% CI from the normal distribution was used to set the cut-off value for amplification above 1.96 and for the deletion below -1.96.