Figure 4
From: Endothelial cells stimulate growth of normal and cancerous breast epithelial cells in 3D culture
BRENCs enhance cloning efficiency of normal and malignant breast epithelial cell lines in co-culture. A) Colony formation (cloning efficiency) is greatly increased in co-cultures. MCF10A cells' colony forming ratio increased from 1% to 41.9%. MDA-MB-231 colony formation increased from 3.2% to 28.6%. Overall, increase in colony formation was greater for the normal cell lines than for the cancer cell lines. B) Density of BRENCs determines the cloning efficiency of MCF10A cells in co-culture. Increasing the density of BRENCs in co-culture resulted in an increase in colony formation (triangle). Colony size reached an apparent plateau at a BRENCs density of 50,000 (square box).